7KES
Crystal structure of meta-AAC0038, an environmental aminoglycoside resistance enzyme, mutant H168A in complex with apramycin and CoA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Aminoglycoside N(3)-acetyltransferase | polymer | 263 | 28654.3 | 2 | UniProt (A0A059X981) | uncultured bacterium | |
| 2 | C, G, H, I (A, B) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 3 | D, J (A, B) | COENZYME A | non-polymer | 767.5 | 2 | Chemie (COA) | |||
| 4 | E, K (A, B) | APRAMYCIN | non-polymer | 539.6 | 2 | Chemie (AM2) | |||
| 5 | F (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | L, M (A, B) | water | water | 18.0 | 167 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 263 (UniProt: A0A059X981)
| PDB | External Database | Details |
|---|---|---|
| Val 1 | - | expression tag |
| Ala 168 | His 168 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 57308.7 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 3018.1 | |
| All* | Total formula weight | 60326.8 |
