7KDV
Murine core lysosomal multienzyme complex (LMC) composed of acid beta-galactosidase (GLB1) and protective protein cathepsin A (PPCA, CTSA)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G, I... | Beta-galactosidase | polymer | 630 | 71450.2 | 6 | UniProt (P23780) Pfam (PF01301) Pfam (PF21317) Pfam (PF21467) In PDB | Mus musculus (Mouse) | Acid beta-galactosidase,Lactase |
| 2 | B, D, F, H, J... | Lysosomal protective protein | polymer | 461 | 52657.1 | 6 | UniProt (P16675) Pfam (PF00450) In PDB | Mus musculus (Mouse) | Carboxypeptidase C,Carboxypeptidase L,Cathepsin A,Protective protein cathepsin A,PPCA,Protective protein for beta-galactosidase |
| 3 | b, c, M, N, P... | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 12 | In PDB GlyTouCan (G42666HT) | |||
| 4 | a, d, O, R, U... | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 6 | In PDB GlyTouCan (G15407YE) | |||
| 5 | I, J, K, A, L... | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 30 | Chemie (NAG) |
Sequence modifications
A, C, E, G, I, K: 28 - 647 (UniProt: P23780)
B, D, F, H, J, L: 24 - 474 (UniProt: P16675)
| PDB | External Database | Details |
|---|---|---|
| Asp 18 | - | expression tag |
| Arg 19 | - | expression tag |
| His 20 | - | expression tag |
| His 21 | - | expression tag |
| His 22 | - | expression tag |
| His 23 | - | expression tag |
| His 24 | - | expression tag |
| His 25 | - | expression tag |
| Gly 26 | - | expression tag |
| Ser 27 | - | expression tag |
| Gln 468 | Arg 468 | engineered mutation |
| Asp 517 | Asn 517 | engineered mutation |
| Gly 534 | Glu 534 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asp 14 | - | expression tag |
| Arg 15 | - | expression tag |
| His 16 | - | expression tag |
| His 17 | - | expression tag |
| His 18 | - | expression tag |
| His 19 | - | expression tag |
| His 20 | - | expression tag |
| His 21 | - | expression tag |
| Gly 22 | - | expression tag |
| Ser 23 | - | expression tag |
| Ala 32 | Cys 32 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 744643.9 | |
| Branched | Number of molecules | 18 |
| Total formula weight | 8612.1 | |
| Non-Polymers* | Number of molecules | 30 |
| Total formula weight | 6636.2 | |
| All* | Total formula weight | 759892.2 |
