Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7KDV

Murine core lysosomal multienzyme complex (LMC) composed of acid beta-galactosidase (GLB1) and protective protein cathepsin A (PPCA, CTSA)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0004565	molecular_function	beta-galactosidase activity
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005737	cellular_component	cytoplasm
A	0005764	cellular_component	lysosome
A	0005773	cellular_component	vacuole
A	0005794	cellular_component	Golgi apparatus
A	0005975	biological_process	carbohydrate metabolic process
A	0006516	biological_process	glycoprotein catabolic process
A	0006689	biological_process	ganglioside catabolic process
A	0016020	cellular_component	membrane
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0016936	molecular_function	galactoside binding
A	0019388	biological_process	galactose catabolic process
A	0042340	biological_process	keratan sulfate proteoglycan catabolic process
A	0042803	molecular_function	protein homodimerization activity
A	0051413	biological_process	response to cortisone
A	1904016	biological_process	response to Thyroglobulin triiodothyronine
B	0004180	molecular_function	carboxypeptidase activity
B	0004185	molecular_function	serine-type carboxypeptidase activity
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0005764	cellular_component	lysosome
B	0006508	biological_process	proteolysis
B	0008233	molecular_function	peptidase activity
B	0016787	molecular_function	hydrolase activity
B	0031647	biological_process	regulation of protein stability
B	1904715	biological_process	negative regulation of chaperone-mediated autophagy
C	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
C	0004565	molecular_function	beta-galactosidase activity
C	0005576	cellular_component	extracellular region
C	0005615	cellular_component	extracellular space
C	0005737	cellular_component	cytoplasm
C	0005764	cellular_component	lysosome
C	0005773	cellular_component	vacuole
C	0005794	cellular_component	Golgi apparatus
C	0005975	biological_process	carbohydrate metabolic process
C	0006516	biological_process	glycoprotein catabolic process
C	0006689	biological_process	ganglioside catabolic process
C	0016020	cellular_component	membrane
C	0016787	molecular_function	hydrolase activity
C	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
C	0016936	molecular_function	galactoside binding
C	0019388	biological_process	galactose catabolic process
C	0042340	biological_process	keratan sulfate proteoglycan catabolic process
C	0042803	molecular_function	protein homodimerization activity
C	0051413	biological_process	response to cortisone
C	1904016	biological_process	response to Thyroglobulin triiodothyronine
D	0004180	molecular_function	carboxypeptidase activity
D	0004185	molecular_function	serine-type carboxypeptidase activity
D	0005515	molecular_function	protein binding
D	0005739	cellular_component	mitochondrion
D	0005764	cellular_component	lysosome
D	0006508	biological_process	proteolysis
D	0008233	molecular_function	peptidase activity
D	0016787	molecular_function	hydrolase activity
D	0031647	biological_process	regulation of protein stability
D	1904715	biological_process	negative regulation of chaperone-mediated autophagy
E	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
E	0004565	molecular_function	beta-galactosidase activity
E	0005576	cellular_component	extracellular region
E	0005615	cellular_component	extracellular space
E	0005737	cellular_component	cytoplasm
E	0005764	cellular_component	lysosome
E	0005773	cellular_component	vacuole
E	0005794	cellular_component	Golgi apparatus
E	0005975	biological_process	carbohydrate metabolic process
E	0006516	biological_process	glycoprotein catabolic process
E	0006689	biological_process	ganglioside catabolic process
E	0016020	cellular_component	membrane
E	0016787	molecular_function	hydrolase activity
E	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
E	0016936	molecular_function	galactoside binding
E	0019388	biological_process	galactose catabolic process
E	0042340	biological_process	keratan sulfate proteoglycan catabolic process
E	0042803	molecular_function	protein homodimerization activity
E	0051413	biological_process	response to cortisone
E	1904016	biological_process	response to Thyroglobulin triiodothyronine
F	0004180	molecular_function	carboxypeptidase activity
F	0004185	molecular_function	serine-type carboxypeptidase activity
F	0005515	molecular_function	protein binding
F	0005739	cellular_component	mitochondrion
F	0005764	cellular_component	lysosome
F	0006508	biological_process	proteolysis
F	0008233	molecular_function	peptidase activity
F	0016787	molecular_function	hydrolase activity
F	0031647	biological_process	regulation of protein stability
F	1904715	biological_process	negative regulation of chaperone-mediated autophagy
G	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
G	0004565	molecular_function	beta-galactosidase activity
G	0005576	cellular_component	extracellular region
G	0005615	cellular_component	extracellular space
G	0005737	cellular_component	cytoplasm
G	0005764	cellular_component	lysosome
G	0005773	cellular_component	vacuole
G	0005794	cellular_component	Golgi apparatus
G	0005975	biological_process	carbohydrate metabolic process
G	0006516	biological_process	glycoprotein catabolic process
G	0006689	biological_process	ganglioside catabolic process
G	0016020	cellular_component	membrane
G	0016787	molecular_function	hydrolase activity
G	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
G	0016936	molecular_function	galactoside binding
G	0019388	biological_process	galactose catabolic process
G	0042340	biological_process	keratan sulfate proteoglycan catabolic process
G	0042803	molecular_function	protein homodimerization activity
G	0051413	biological_process	response to cortisone
G	1904016	biological_process	response to Thyroglobulin triiodothyronine
H	0004180	molecular_function	carboxypeptidase activity
H	0004185	molecular_function	serine-type carboxypeptidase activity
H	0005515	molecular_function	protein binding
H	0005739	cellular_component	mitochondrion
H	0005764	cellular_component	lysosome
H	0006508	biological_process	proteolysis
H	0008233	molecular_function	peptidase activity
H	0016787	molecular_function	hydrolase activity
H	0031647	biological_process	regulation of protein stability
H	1904715	biological_process	negative regulation of chaperone-mediated autophagy
I	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
I	0004565	molecular_function	beta-galactosidase activity
I	0005576	cellular_component	extracellular region
I	0005615	cellular_component	extracellular space
I	0005737	cellular_component	cytoplasm
I	0005764	cellular_component	lysosome
I	0005773	cellular_component	vacuole
I	0005794	cellular_component	Golgi apparatus
I	0005975	biological_process	carbohydrate metabolic process
I	0006516	biological_process	glycoprotein catabolic process
I	0006689	biological_process	ganglioside catabolic process
I	0016020	cellular_component	membrane
I	0016787	molecular_function	hydrolase activity
I	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
I	0016936	molecular_function	galactoside binding
I	0019388	biological_process	galactose catabolic process
I	0042340	biological_process	keratan sulfate proteoglycan catabolic process
I	0042803	molecular_function	protein homodimerization activity
I	0051413	biological_process	response to cortisone
I	1904016	biological_process	response to Thyroglobulin triiodothyronine
J	0004180	molecular_function	carboxypeptidase activity
J	0004185	molecular_function	serine-type carboxypeptidase activity
J	0005515	molecular_function	protein binding
J	0005739	cellular_component	mitochondrion
J	0005764	cellular_component	lysosome
J	0006508	biological_process	proteolysis
J	0008233	molecular_function	peptidase activity
J	0016787	molecular_function	hydrolase activity
J	0031647	biological_process	regulation of protein stability
J	1904715	biological_process	negative regulation of chaperone-mediated autophagy
K	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
K	0004565	molecular_function	beta-galactosidase activity
K	0005576	cellular_component	extracellular region
K	0005615	cellular_component	extracellular space
K	0005737	cellular_component	cytoplasm
K	0005764	cellular_component	lysosome
K	0005773	cellular_component	vacuole
K	0005794	cellular_component	Golgi apparatus
K	0005975	biological_process	carbohydrate metabolic process
K	0006516	biological_process	glycoprotein catabolic process
K	0006689	biological_process	ganglioside catabolic process
K	0016020	cellular_component	membrane
K	0016787	molecular_function	hydrolase activity
K	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
K	0016936	molecular_function	galactoside binding
K	0019388	biological_process	galactose catabolic process
K	0042340	biological_process	keratan sulfate proteoglycan catabolic process
K	0042803	molecular_function	protein homodimerization activity
K	0051413	biological_process	response to cortisone
K	1904016	biological_process	response to Thyroglobulin triiodothyronine
L	0004180	molecular_function	carboxypeptidase activity
L	0004185	molecular_function	serine-type carboxypeptidase activity
L	0005515	molecular_function	protein binding
L	0005739	cellular_component	mitochondrion
L	0005764	cellular_component	lysosome
L	0006508	biological_process	proteolysis
L	0008233	molecular_function	peptidase activity
L	0016787	molecular_function	hydrolase activity
L	0031647	biological_process	regulation of protein stability
L	1904715	biological_process	negative regulation of chaperone-mediated autophagy

Functional Information from PROSITE/UniProt

site_id	PS01182
Number of Residues	13
Details	GLYCOSYL_HYDROL_F35 Glycosyl hydrolases family 35 putative active site. GGPIItvQvENEY

Chain	Residue	Details
A	GLY178-TYR190

site_id	PS00131
Number of Residues	8
Details	CARBOXYPEPT_SER_SER Serine carboxypeptidases, serine active site. LtGESYAG

Chain	Residue	Details
B	LEU169-GLY176

site_id	PS00560
Number of Residues	18
Details	CARBOXYPEPT_SER_HIS Serine carboxypeptidases, histidine active site. ItfLtIkGAGHmVPtdkP

Chain	Residue	Details
B	ILE441-PRO458

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	Active site: {"description":"Proton donor","evidences":[{"source":"UniProtKB","id":"P16278","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	6
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"UniProtKB","id":"P16278","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P16278","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	36
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	18
Details	Active site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	12
Details	Glycosylation: {"description":"N-linked (GlcNAc...) (high mannose) asparagine","evidences":[{"source":"PubMed","id":"16170054","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)