7K5J
Structure of an E1-E2-ubiquitin thioester mimetic
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, N, O, P, Q... (N, M, O, P, Q...) | Ubiquitin | polymer | 83 | 9692.9 | 8 | UniProt (P69326) Pfam (PF00240) | Triticum aestivum (Wheat) | |
| 2 | B, D, F, H, J... (D, A, C, G, I...) | Ubiquitin-activating enzyme E1 1 | polymer | 1017 | 113704.2 | 8 | UniProt (P22515) Pfam (PF00899) Pfam (PF16190) Pfam (PF16191) Pfam (PF10585) Pfam (PF09358) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 3 | C, E, G, I, K... (F, B, E, H, J...) | Ubiquitin-conjugating enzyme E2-34 kDa | polymer | 197 | 22485.3 | 8 | UniProt (P14682) Pfam (PF00179) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Cell division control protein 34,E2 ubiquitin-conjugating enzyme 3,E3 ubiquitin ligase complex SCF subunit CDC34,Ubiquitin carrier protein,Ubiquitin-protein ligase |
| 4 | AA, BA, CA, DA, EA... (C, G, I, K, S...) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 7 | Chemie (AMP) |
Sequence modifications
N, M, O, P, Q, R, W, X: 1 - 76 (UniProt: P69326)
D, A, C, G, I, K, S, U: 11 - 1024 (UniProt: P22515)
F, B, E, H, J, L, T, V: 3 - 195 (UniProt: P14682)
| PDB | External Database | Details |
|---|---|---|
| Met -6 | - | initiating methionine |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| Arg 6 | Lys 6 | conflict |
| Arg 11 | Lys 11 | conflict |
| Arg 27 | Lys 27 | conflict |
| Arg 29 | Lys 29 | conflict |
| Arg 33 | Lys 33 | conflict |
| Arg 48 | Lys 48 | conflict |
| Arg 63 | Lys 63 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 8 | - | expression tag |
| Ala 9 | - | expression tag |
| Met 10 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Met 1 | - | expression tag |
| Ala 2 | - | expression tag |
| Lys 141 | Ala 141 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 24 |
| Total formula weight | 1167059.3 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 2430.5 | |
| All* | Total formula weight | 1169489.8 |
