7K04
Structure of TFIIH/Rad4-Rad23-Rad33/DNA in DNA opening
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (E) | DNA repair protein RAD33 | polymer | 177 | 20291.5 | 1 | UniProt (Q04231) Pfam (PF08730) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 2 | B (2) | General transcription and DNA repair factor IIH subunit TFB2 | polymer | 513 | 58602.3 | 1 | UniProt (Q02939) Pfam (PF03849) Pfam (PF18307) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIH subunit TFB2,RNA polymerase II transcription factor B 52 kDa subunit,RNA polymerase II transcription factor B p52 subunit,RNA polymerase II transcription factor B subunit 2 |
| 3 | C (0) | DNA repair helicase RAD3 | polymer | 778 | 89899.0 | 1 | UniProt (P06839) Pfam (PF06733) Pfam (PF06777) Pfam (PF13307) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | General transcription and DNA repair factor IIH subunit RAD3,TFIIH subunit RAD3 |
| 4 | D (1) | General transcription and DNA repair factor IIH subunit TFB1 | polymer | 642 | 72959.3 | 1 | UniProt (P32776) Pfam (PF08567) Pfam (PF03909) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIH subunit TFB1,RNA polymerase II transcription factor B 73 kDa subunit,RNA polymerase II transcription factor B p73 subunit,RNA polymerase II transcription factor B subunit 1 |
| 5 | E (4) | General transcription and DNA repair factor IIH subunit TFB4 | polymer | 338 | 37506.4 | 1 | UniProt (Q12004) Pfam (PF03850) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIH subunit TFB4,RNA polymerase II transcription factor B 34 kDa subunit,RNA polymerase II transcription factor B p34 subunit,RNA polymerase II transcription factor B subunit 4 |
| 6 | F (6) | General transcription and DNA repair factor IIH subunit SSL1 | polymer | 461 | 52370.0 | 1 | UniProt (Q04673) Pfam (PF04056) Pfam (PF07975) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIH subunit SSL1,RNA polymerase II transcription factor B subunit SSL1,TFB subunit SSL1,Suppressor of stem-loop protein 1 |
| 7 | G (Y) | Damaged DNA strand | polymer | 27 | 8641.6 | 1 | synthetic construct | ||
| 8 | H (W) | Undamaged DNA strand | polymer | 29 | 8855.8 | 1 | synthetic construct | ||
| 9 | I (A) | DNA repair protein RAD4 | polymer | 754 | 87374.5 | 1 | UniProt (P14736) Pfam (PF03835) Pfam (PF10403) Pfam (PF10405) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 10 | J (7) | DNA repair helicase RAD25 | polymer | 843 | 95461.7 | 1 | UniProt (Q00578) Pfam (PF13625) Pfam (PF04851) Pfam (PF16203) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | General transcription and DNA repair factor IIH subunit RAD25,TFIIH subunit RAD25,Suppressor of stem-loop mutation 2 |
| 11 | K (5) | RNA polymerase II transcription factor B subunit 5 | polymer | 72 | 8243.5 | 1 | UniProt (Q3E7C1) Pfam (PF06331) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | General transcription and DNA repair factor IIH subunit TFB5,TFIIH subunit TFB5 |
| 12 | L, M (E) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 13 | N (0) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) | |||
| 14 | O, P, Q, R, S (4, 6) | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) |
Sequence modifications
1: 2 - 642 (UniProt: P32776)
A: 1 - 754 (UniProt: P14736)
| PDB | External Database | Details |
|---|---|---|
| Pro 1 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Glu 223 | Val 223 | conflict |
| Arg 427 | Gln 427 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 540205.5 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 758.8 | |
| All* | Total formula weight | 540964.3 |
