7K04
Structure of TFIIH/Rad4-Rad23-Rad33/DNA in DNA opening
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM CPC |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 9.25 |
| Software | RELION,Latitude,CTFFIND |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
