7JVR
Cryo-EM structure of Bromocriptine-bound dopamine receptor 2 in complex with Gi protein
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | R | Soluble cytochrome b562,D(2) dopamine receptor | polymer | 588 | 67234.6 | 1 | UniProt (P0ABE7) UniProt (P14416) Pfam (PF07361) Pfam (PF00001) In PDB | Escherichia coli | Cytochrome b-562,Dopamine D2 receptor |
2 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
3 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 348 | 38146.7 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (Human) | Transducin beta chain 1 |
4 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (Human) | G gamma-I |
5 | E | Antibody fragment ScFv16 | polymer | 269 | 28813.0 | 1 | synthetic construct | ||
6 | R | bromoergocryptine | non-polymer | 654.6 | 1 | Chemie (08Y) |
Sequence modifications
R: -116 - -12 (UniProt: P0ABE7)
R: 1 - 443 (UniProt: P14416)
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
PDB | External Database | Details |
---|---|---|
Asp -144 | - | expression tag |
Tyr -143 | - | expression tag |
Lys -142 | - | expression tag |
Asp -141 | - | expression tag |
Asp -140 | - | expression tag |
Asp -139 | - | expression tag |
Asp -138 | - | expression tag |
Ala -137 | - | expression tag |
Lys -136 | - | expression tag |
Leu -135 | - | expression tag |
Gln -134 | - | expression tag |
Thr -133 | - | expression tag |
Met -132 | - | expression tag |
His -131 | - | expression tag |
His -130 | - | expression tag |
His -129 | - | expression tag |
His -128 | - | expression tag |
His -127 | - | expression tag |
His -126 | - | expression tag |
His -125 | - | expression tag |
His -124 | - | expression tag |
His -123 | - | expression tag |
His -122 | - | expression tag |
His -121 | - | expression tag |
His -120 | - | expression tag |
His -119 | - | expression tag |
His -118 | - | expression tag |
His -117 | - | expression tag |
Trp -110 | Met 29 | conflict |
Ile -15 | His 124 | conflict |
Leu -11 | - | linker |
Ala -10 | - | linker |
Ser -9 | - | linker |
Glu -8 | - | linker |
Asn -7 | - | linker |
Leu -6 | - | linker |
Tyr -5 | - | linker |
Phe -4 | - | linker |
Gln -3 | - | linker |
Gly -2 | - | linker |
Gly -1 | - | linker |
Thr 0 | - | linker |
A: 1 - 354 (UniProt: P63096)
PDB | External Database | Details |
---|---|---|
Asn 47 | Ser 47 | engineered mutation |
Ala 203 | Gly 203 | engineered mutation |
Ala 245 | Glu 245 | engineered mutation |
Ser 326 | Ala 326 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -4 | - | initiating methionine |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Gly 341 | - | expression tag |
Ser 342 | - | expression tag |
Ser 343 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 182469.5 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 654.6 | |
All* | Total formula weight | 183124.1 |