7JSN
Structure of the Visual Signaling Complex between Transducin and Phosphodiesterase 6
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha | polymer | 859 | 99461.8 | 1 | UniProt (P11541) Pfam (PF01590) Pfam (PF00233) In PDB | Bos taurus (Bovine) | GMP-PDE alpha,PDE V-B1 |
2 | B | Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta | polymer | 853 | 98449.6 | 1 | UniProt (P23439) Pfam (PF01590) Pfam (PF00233) In PDB | Bos taurus (Bovine) | GMP-PDE beta |
3 | E, F | Guanine nucleotide-binding protein G(t) subunit alpha-1, Guanine nucleotide-binding protein G(i) subunit alpha-1 chimera | polymer | 371 | 42218.0 | 2 | UniProt (P04695) Pfam (PF00503) In PDB | Bos taurus (Bovine) | Transducin alpha-1 chain,Transducin alpha-1 chain |
4 | C, D | Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma | polymer | 87 | 9684.2 | 2 | UniProt (P04972) Pfam (PF04868) In PDB | Bos taurus (Bovine) | GMP-PDE gamma |
5 | A, B | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
6 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
7 | A, B | 2-{2-ETHOXY-5-[(4-ETHYLPIPERAZIN-1-YL)SULFONYL]PHENYL}-5-METHYL-7-PROPYLIMIDAZO[5,1-F][1,2,4]TRIAZIN-4(1H)-ONE | non-polymer | 488.6 | 2 | Chemie (VDN) | |||
8 | A, B | GUANOSINE-3',5'-MONOPHOSPHATE | non-polymer | 345.2 | 2 | Chemie (35G) | |||
9 | E, F | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) |
Sequence modifications
E, F: 1 - 215 (UniProt: P04695)
E, F: 216 - 294 (PDB: 7JSN)
E, F: 295 - 350 (UniProt: P04695)
PDB | External Database | Details |
---|---|---|
Met -8 | - | initiating methionine |
Ala -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
Ala 0 | - | expression tag |
Cys 174 | Arg 174 | engineered mutation |
Leu 200 | Gln 200 | engineered mutation |
E, F: 295 - 350 (UniProt: P04695)
PDB | External Database | Details |
---|---|---|
Ala 351 | - | expression tag |
Ala 352 | - | expression tag |
Ala 353 | - | expression tag |
Thr 354 | - | expression tag |
Glu 355 | - | expression tag |
Thr 356 | - | expression tag |
Ser 357 | - | expression tag |
Gln 358 | - | expression tag |
Val 359 | - | expression tag |
Ala 360 | - | expression tag |
Pro 361 | - | expression tag |
Ala 362 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 301716.0 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 2893.4 | |
All* | Total formula weight | 304609.4 |