7JSN
Structure of the Visual Signaling Complex between Transducin and Phosphodiesterase 6
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha | polymer | 859 | 99461.8 | 1 | UniProt (P11541) Pfam (PF01590) Pfam (PF00233) | Bos taurus (Bovine) | GMP-PDE alpha,PDE V-B1 |
| 2 | B (B) | Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta | polymer | 853 | 98449.6 | 1 | UniProt (P23439) Pfam (PF01590) Pfam (PF00233) | Bos taurus (Bovine) | GMP-PDE beta |
| 3 | C, D (E, F) | Guanine nucleotide-binding protein G(t) subunit alpha-1, Guanine nucleotide-binding protein G(i) subunit alpha-1 chimera | polymer | 371 | 42218.0 | 2 | UniProt (P04695) Pfam (PF00503) | Bos taurus (Bovine) | Transducin alpha-1 chain,Transducin alpha-1 chain |
| 4 | E, F (C, D) | Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma | polymer | 87 | 9684.2 | 2 | UniProt (P04972) Pfam (PF04868) | Bos taurus (Bovine) | GMP-PDE gamma |
| 5 | G, K (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 6 | H, L (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 7 | I, M (A, B) | 2-{2-ETHOXY-5-[(4-ETHYLPIPERAZIN-1-YL)SULFONYL]PHENYL}-5-METHYL-7-PROPYLIMIDAZO[5,1-F][1,2,4]TRIAZIN-4(1H)-ONE | non-polymer | 488.6 | 2 | Chemie (VDN) | |||
| 8 | J, N (A, B) | GUANOSINE-3',5'-MONOPHOSPHATE | non-polymer | 345.2 | 2 | Chemie (35G) | |||
| 9 | O, P (E, F) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) |
Sequence modifications
E, F: 1 - 215 (UniProt: P04695)
E, F: 216 - 294 (PDB: 7JSN)
E, F: 295 - 350 (UniProt: P04695)
| PDB | External Database | Details |
|---|---|---|
| Met -8 | - | initiating methionine |
| Ala -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Cys 174 | Arg 174 | engineered mutation |
| Leu 200 | Gln 200 | engineered mutation |
E, F: 295 - 350 (UniProt: P04695)
| PDB | External Database | Details |
|---|---|---|
| Ala 351 | - | expression tag |
| Ala 352 | - | expression tag |
| Ala 353 | - | expression tag |
| Thr 354 | - | expression tag |
| Glu 355 | - | expression tag |
| Thr 356 | - | expression tag |
| Ser 357 | - | expression tag |
| Gln 358 | - | expression tag |
| Val 359 | - | expression tag |
| Ala 360 | - | expression tag |
| Pro 361 | - | expression tag |
| Ala 362 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 301716.0 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2893.4 | |
| All* | Total formula weight | 304609.4 |
