7JSN
Structure of the Visual Signaling Complex between Transducin and Phosphodiesterase 6
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha | polymer | 859 | 99461.8 | 1 | UniProt (P11541) Pfam (PF01590) Pfam (PF00233) | Bos taurus (Bovine) | GMP-PDE alpha,PDE V-B1 |
| 2 | B (B) | Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta | polymer | 853 | 98449.6 | 1 | UniProt (P23439) Pfam (PF01590) Pfam (PF00233) | Bos taurus (Bovine) | GMP-PDE beta |
| 3 | C, D (E, F) | Guanine nucleotide-binding protein G(t) subunit alpha-1, Guanine nucleotide-binding protein G(i) subunit alpha-1 chimera | polymer | 371 | 42218.0 | 2 | UniProt (P04695) Pfam (PF00503) | Bos taurus (Bovine) | Transducin alpha-1 chain,Transducin alpha-1 chain |
| 4 | E, F (C, D) | Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma | polymer | 87 | 9684.2 | 2 | UniProt (P04972) Pfam (PF04868) | Bos taurus (Bovine) | GMP-PDE gamma |
| 5 | G, K (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 6 | H, L (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | I, M (A, B) | 2-{2-ETHOXY-5-[(4-ETHYLPIPERAZIN-1-YL)SULFONYL]PHENYL}-5-METHYL-7-PROPYLIMIDAZO[5,1-F][1,2,4]TRIAZIN-4(1H)-ONE | non-polymer | 488.6 | 2 | Chemie (VDN) | |||
| 8 | J, N (A, B) | GUANOSINE-3',5'-MONOPHOSPHATE | non-polymer | 345.2 | 2 | Chemie (35G) | |||
| 9 | O, P (E, F) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) |
Sequence modifications
E, F: 1 - 215 (UniProt: P04695)
E, F: 216 - 294 (PDB: 7JSN)
E, F: 295 - 350 (UniProt: P04695)
| PDB | External Database | Details |
|---|---|---|
| Met -8 | - | initiating methionine |
| Ala -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Cys 174 | Arg 174 | engineered mutation |
| Leu 200 | Gln 200 | engineered mutation |
E, F: 295 - 350 (UniProt: P04695)
| PDB | External Database | Details |
|---|---|---|
| Ala 351 | - | expression tag |
| Ala 352 | - | expression tag |
| Ala 353 | - | expression tag |
| Thr 354 | - | expression tag |
| Glu 355 | - | expression tag |
| Thr 356 | - | expression tag |
| Ser 357 | - | expression tag |
| Gln 358 | - | expression tag |
| Val 359 | - | expression tag |
| Ala 360 | - | expression tag |
| Pro 361 | - | expression tag |
| Ala 362 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 301716.0 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2893.4 | |
| All* | Total formula weight | 304609.4 |
