7JS5
Phospholipase D engineered mutant (TNYR) inactive enzyme (H168A) bound to 1-inositol phosphate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Phospholipase D | polymer | 509 | 53848.8 | 1 | UniProt (Q53728) Pfam (PF13091) | Streptomyces antibioticus | Choline phosphatase |
| 2 | B (A) | D-MYO-INOSITOL-1-PHOSPHATE | non-polymer | 258.1 | 1 | Chemie (IPD) | |||
| 3 | C (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 4 | D (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | E (A) | water | water | 18.0 | 140 | Chemie (HOH) |
Sequence modifications
A: 1 - 509 (UniProt: Q53728)
| PDB | External Database | Details |
|---|---|---|
| Ser 107 | Val 154 | engineered mutation |
| Ala 168 | His 215 | engineered mutation |
| Thr 186 | Gly 233 | engineered mutation |
| Asn 187 | Trp 234 | engineered mutation |
| Arg 211 | Ala 258 | engineered mutation |
| Ala 377 | Arg 424 | conflict |
| Arg 385 | Tyr 432 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 53848.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 545.5 | |
| All* | Total formula weight | 54394.3 |
