7JOZ

Crystal structure of dopamine D1 receptor in complex with G protein and a non-catechol agonist

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	380	44326.2	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (N)	Nanobody 35	polymer	160	17352.5	1		Lama glama
5	E (R)	Endolysin,D(1A) dopamine receptor	polymer	540	59856.9	1	UniProt (D9IEF7) UniProt (P21728) Pfam (PF00959) Pfam (PF00001)	Enterobacteria phage T4 (Bacteriophage T4)	Lysis protein,Lysozyme,Muramidase,Dopamine D1 receptor
6	F (R)	6-{4-[(furo[3,2-c]pyridin-4-yl)oxy]-2-methylphenyl}-1,5-dimethylpyrimidine-2,4(1H,3H)-dione	non-polymer		363.4	1	Chemie (VFP)
7	G (R)	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 1002 - 1161 (UniProt: D9IEF7)

PDB	External Database	Details
Asp 956	-	expression tag
Tyr 957	-	expression tag
Lys 958	-	expression tag
Asp 959	-	expression tag
Asp 960	-	expression tag
Asp 961	-	expression tag
Asp 962	-	expression tag
Ala 963	-	expression tag
Ala 964	-	expression tag
Ala 965	-	expression tag
Gly 966	-	expression tag
Gln 967	-	expression tag
Pro 968	-	expression tag
Gly 969	-	expression tag
Asn 970	-	expression tag
Gly 971	-	expression tag
Ser 972	-	expression tag
Ala 973	-	expression tag
Phe 974	-	expression tag
Leu 975	-	expression tag
Leu 976	-	expression tag
Ala 977	-	expression tag
Pro 978	-	expression tag
Asn 979	-	expression tag
Arg 980	-	expression tag
Ser 981	-	expression tag
His 982	-	expression tag
Ala 983	-	expression tag
Pro 984	-	expression tag
Gly 985	-	expression tag
Gly 986	-	expression tag
Gly 987	-	expression tag
Gly 988	-	expression tag
Ser 989	-	expression tag
Leu 990	-	expression tag
Glu 991	-	expression tag
Val 992	-	expression tag
Leu 993	-	expression tag
Phe 994	-	expression tag
Gln 995	-	expression tag
Gly 996	-	expression tag
Pro 997	-	expression tag
Gly 998	-	expression tag
Gly 999	-	expression tag
Gly 1000	-	expression tag
Ser 1001	-	expression tag
Thr 1054	Cys 54	conflict
Ala 1097	Cys 97	conflict
Ala 1162	-	linker

R: 21 - 347 (UniProt: P21728)

PDB	External Database	Details
His 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	167930.8
Non-Polymers*	Number of molecules	2
	Total formula weight	601.6
All*	Total formula weight	168532.5

*Water molecules are not included.