7JJO
Structural Basis of the Activation of Heterotrimeric Gs-protein by Isoproterenol-bound Beta1-Adrenergic Receptor
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 339 | 37285.7 | 1 | UniProt (P62871) Pfam (PF00400) | Bos taurus (Bovine) | Transducin beta chain 1 |
| 2 | B (N) | Nanobody 35 | polymer | 138 | 15140.7 | 1 | Lama glama (llama) | ||
| 3 | C (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 384 | 44694.6 | 1 | UniProt (P04896) Pfam (PF00503) | Bos taurus (Bovine) | Adenylate cyclase-stimulating G alpha protein |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7845.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (Bovine) | G gamma-I |
| 5 | E (R) | Beta1-Adrenergic Receptor | polymer | 508 | 57958.7 | 1 | Pfam (PF00001) UniProt (by SIFTS) (P07700) | Meleagris gallopavo | |
| 6 | F (R) | ISOPRENALINE | non-polymer | 211.3 | 1 | Chemie (5FW) |
Sequence modifications
A: 1 - 380 (UniProt: P04896)
G: 1 - 71 (UniProt: P63212)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Gly 18 | Ala 18 | conflict |
| Ser 72 | Gly 72 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser 68 | Cys 68 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 162924.8 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 211.3 | |
| All* | Total formula weight | 163136.0 |
