7JIB
Room Temperature Crystal Structure of Nsp10/Nsp16 from SARS-CoV-2 with Substrates and Products of 2'-O-methylation of the Cap-1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 2'-O-methyltransferase | polymer | 301 | 33627.5 | 1 | UniProt (P0DTD1) Pfam (PF06460) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) | Non-structural protein 16 |
| 2 | B (B) | Non-structural protein 10 | polymer | 142 | 15075.2 | 1 | UniProt (P0DTD1) Pfam (PF09401) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) | |
| 3 | C, D (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) PubChem (312) | |||
| 4 | E (A) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (145712080) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (5136) PubChem (34755) PubChem (9865603) PubChem (12312798) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094) | |||
| 5 | F (A) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) PubChem (16760024) PubChem (25210495) PubChem (177678424) PubChem (21125603) PubChem (25203782) PubChem (52224400) PubChem (44361184) PubChem (46936206) PubChem (50989525) PubChem (51380914) PubChem (6419189) PubChem (52224398) PubChem (57544532) PubChem (164157219) PubChem (59046843) PubChem (59170628) PubChem (59576228) PubChem (66805708) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (118374891) PubChem (118560068) PubChem (193) PubChem (13792) PubChem (189149) PubChem (439155) PubChem (5284331) PubChem (126969526) PubChem (71170554) PubChem (129105892) PubChem (13231890) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525) | |||
| 6 | G (A) | P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE | non-polymer | 787.4 | 1 | Chemie (GTA) | |||
| 7 | H (A) | 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-ADENOSINE | non-polymer | 801.5 | 1 | Chemie (V9G) | |||
| 8 | I (A) | 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 538.2 | 1 | Chemie (MGP) | |||
| 9 | J, K (B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | L, M (A, B) | water | water | 18.0 | 141 | Chemie (HOH) |
Sequence modifications
A: 1 - 298 (UniProt: P0DTD1)
B: 1 - 139 (UniProt: P0DTD1)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 48702.7 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 3111.7 | |
| All* | Total formula weight | 51814.4 |
