7FIY
Cryo-EM structure of the tirzepatide-bound human GIPR-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Gastric inhibitory polypeptide receptor,Gastric inhibitory polypeptide receptor,human glucose-dependent insulinotropic polypeptide receptor | polymer | 573 | 64950.7 | 1 | UniProt (P48546) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (Human) | GIP-R,Glucose-dependent insulinotropic polypeptide receptor |
| 2 | B (P) | Tiezepatide | polymer | 39 | 4072.5 | 1 | synthetic construct | ||
| 3 | C (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45743.4 | 1 | UniProt (P04896) Pfam (PF00503) | Bos taurus (Bovine) | Adenylate cyclase-stimulating G alpha protein |
| 4 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 371 | 40227.0 | 1 | UniProt (P54311) Pfam (PF00400) | Rattus norvegicus (Rat) | Transducin beta chain 1 |
| 5 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (Bovine) | G gamma-I |
| 6 | F (N) | Nanobody-35 | polymer | 140 | 15343.0 | 1 | synthetic construct | ||
| 7 | G, H, I, J, K... (R) | CHOLESTEROL | non-polymer | 386.7 | 6 | Chemie (CLR) |
Sequence modifications
R: 22 - 421 (UniProt: P48546)
R: 422 - 594 (PDB: 7FIY)
A: 1 - 394 (UniProt: P04896)
B: 2 - 340 (UniProt: P54311)
| PDB | External Database | Details |
|---|---|---|
| Phe 345 | Thr 345 | engineered mutation |
A: 1 - 394 (UniProt: P04896)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| Ser 366 | Ala 366 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ser 343 | - | expression tag |
| Gly 344 | - | expression tag |
| Gly 345 | - | expression tag |
| Gly 346 | - | expression tag |
| Gly 347 | - | expression tag |
| Ser 348 | - | expression tag |
| Gly 349 | - | expression tag |
| Gly 350 | - | expression tag |
| Gly 351 | - | expression tag |
| Gly 352 | - | expression tag |
| Ser 353 | - | expression tag |
| Ser 354 | - | expression tag |
| Gly 355 | - | expression tag |
| Val 356 | - | expression tag |
| Ser 357 | - | expression tag |
| Gly 358 | - | expression tag |
| Trp 359 | - | expression tag |
| Arg 360 | - | expression tag |
| Leu 361 | - | expression tag |
| Phe 362 | - | expression tag |
| Lys 363 | - | expression tag |
| Lys 364 | - | expression tag |
| Ile 365 | - | expression tag |
| Ser 366 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 178197.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 2319.9 | |
| All* | Total formula weight | 180517.7 |
