7FIM
Cryo-EM structure of the tirzepatide (LY3298176)-bound human GLP-1R-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (N) | Nanobody-35 | polymer | 140 | 15343.0 | 1 | synthetic construct | ||
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 356 | 39221.1 | 1 | UniProt (P54311) Pfam (PF00400) | Rattus norvegicus (Rat) | Transducin beta chain 1 |
| 3 | C (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45743.4 | 1 | UniProt (P04896) Pfam (PF00503) | Bos taurus (Bovine) | Adenylate cyclase-stimulating G alpha protein |
| 4 | D (R) | Glucagon-like peptide 1 receptor,Glucagon-like peptide 1 receptor,Glucagon-like peptide 1 receptor,Glucagon-like peptide 1 receptor,human glucagon like peptide 1 receptor | polymer | 597 | 68355.7 | 1 | UniProt (P43220) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (Human) | GLP-1 receptor,GLP-1-R,GLP-1R |
| 5 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (Bovine) | G gamma-I |
| 6 | F (P) | Tirzepatide | polymer | 39 | 4072.5 | 1 | Homo sapiens |
Sequence modifications
B: 7 - 345 (UniProt: P54311)
A: 1 - 394 (UniProt: P04896)
R: 24 - 463 (UniProt: P43220)
R: 464 - 620 (PDB: 7FIM)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Gly 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Leu 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Gln 6 | - | expression tag |
| Val 346 | - | expression tag |
| Ser 347 | - | expression tag |
| Gly 348 | - | expression tag |
| Trp 349 | - | expression tag |
| Arg 350 | - | expression tag |
| Leu 351 | - | expression tag |
| Phe 352 | - | expression tag |
| Lys 353 | - | expression tag |
| Lys 354 | - | expression tag |
| Ile 355 | - | expression tag |
| Ser 356 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| Ser 366 | Ala 366 | engineered mutation |
R: 464 - 620 (PDB: 7FIM)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 180596.9 | |
| All* | Total formula weight | 180596.9 |
