7FE4

Crystal structure of GH65 alpha-1,2-glucosidase from Flavobacterium johnsoniae in complex with glucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65	polymer	678	76489.2	3	UniProt (A5FBJ5) Pfam (PF03636) Pfam (PF03632)	Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) (Cytophaga johnsonae)	alpha-1,2-glucosidase
2	D, E, F, G, H... (A, B, C)	beta-D-glucopyranose	non-polymer		180.2	12	Chemie (BGC)
3	P, Q, R (A, B, C)	water	water		18.0	1668	Chemie (HOH)

Sequence modifications

A, B, C: 24 - 681 (UniProt: A5FBJ5)

PDB	External Database	Details
Met 4	-	initiating methionine
Gly 5	-	expression tag
Ser 6	-	expression tag
Ser 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
Ser 14	-	expression tag
Ser 15	-	expression tag
Gly 16	-	expression tag
Leu 17	-	expression tag
Val 18	-	expression tag
Pro 19	-	expression tag
Arg 20	-	expression tag
Gly 21	-	expression tag
Ser 22	-	expression tag
His 23	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	229467.7
Non-Polymers*	Number of molecules	12
	Total formula weight	2161.9
All*	Total formula weight	231629.5

*Water molecules are not included.