7FE2

Crystal structure of the mutant E494Q of GH92 alpha-1,2-mannosidase from Enterococcus faecalis ATCC 10100 in complex with alpha-1,2-mannobiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Alpha-1,2-mannosidase	polymer	721	82733.1	4	UniProt (A0A6N0WQ22) Pfam (PF17678) Pfam (PF07971) UniProt (by SIFTS) (Q832K9) In PDB	Enterococcus faecalis ATCC 10100 (Streptococcus faecalis)
2	E, F, G, H	alpha-D-mannopyranose-(1-2)-beta-D-mannopyranose	branched		342.3	4	In PDB GlyTouCan (G09507TI)
3	C, A, D, B	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
4	C, A, D, B	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
5	B, C, A, D	1,2-ETHANEDIOL	non-polymer		62.1	26	Chemie (EDO)
6	A, B	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
7		water	water		18.0	1773	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 713 (UniProt: A0A6N0WQ22)

PDB	External Database	Details
Gln 494	Glu 494	engineered mutation
Leu 714	-	expression tag
Glu 715	-	expression tag
His 716	-	expression tag
His 717	-	expression tag
His 718	-	expression tag
His 719	-	expression tag
His 720	-	expression tag
His 721	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	330932.3
Branched	Number of molecules	4
	Total formula weight	1369.2
Non-Polymers*	Number of molecules	36
	Total formula weight	1984.1
All*	Total formula weight	334285.6

*Water molecules are not included.