7FE1

Crystal structure of GH92 alpha-1,2-mannosidase from Enterococcus faecalis ATCC 10100 in complex with methyl alpha-1,2-C-mannobioside

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Alpha-1,2-mannosidase	polymer	721	82734.1	4	UniProt (A0A6N0WQ22) Pfam (PF17678) Pfam (PF07971) UniProt (by SIFTS) (Q832K9)	Enterococcus faecalis ATCC 10100 (Streptococcus faecalis)
2	E, HA, O, Z (A, D, B, C)	methyl 2-deoxy-2-methyl-alpha-D-mannopyranoside	non-polymer		192.2	4	Chemie (5II)
3	AA, F, IA, P (C, A, D, B)	alpha-D-mannopyranose	non-polymer		180.2	4	Chemie (MAN)
4	BA, G, JA, Q (C, A, D, B)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
5	CA, H, KA, R (C, A, D, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
6	I, T (A, B)	ACETIC ACID	non-polymer		60.1	2	Chemie (ACY)
7	DA, EA, FA, GA, J... (C, A, D, B)	1,2-ETHANEDIOL	non-polymer		62.1	18	Chemie (EDO)
8	OA, PA, QA, RA (A, B, C, D)	water	water		18.0	2760	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 713 (UniProt: A0A6N0WQ22)

PDB	External Database	Details
Leu 714	-	expression tag
Glu 715	-	expression tag
His 716	-	expression tag
His 717	-	expression tag
His 718	-	expression tag
His 719	-	expression tag
His 720	-	expression tag
His 721	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	330936.3
Non-Polymers*	Number of molecules	36
	Total formula weight	2979.1
All*	Total formula weight	333915.3

*Water molecules are not included.