7FDD

A Crystal structure of OspA mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Outer surface protein A	polymer	245	25605.5	2	UniProt (P0CL66) Pfam (PF00820)	Borreliella burgdorferi
2	C, D (A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)

Sequence modifications

A, B: 27 - 267 (UniProt: P0CL66)

PDB	External Database	Details
Gly 23	-	expression tag
Ser 24	-	expression tag
His 25	-	expression tag
Met 26	-	expression tag
Ser 37	Glu 37	engineered mutation
Ser 45	Glu 45	engineered mutation
Ser 46	Lys 46	engineered mutation
Ala 48	Lys 48	engineered mutation
Ala 60	Lys 60	engineered mutation
Ser 64	Lys 64	engineered mutation
Ala 83	Lys 83	engineered mutation
Ser 104	Glu 104	engineered mutation
Ser 107	Lys 107	engineered mutation
-	Lys 117	deletion
-	Asp 118	deletion
-	Lys 119	deletion
Gly 117	-	insertion
Gly 118	-	insertion
-	Glu 124	deletion
-	Lys 125	deletion
-	Phe 126	deletion
-	Asn 127	deletion
-	Gly 130	deletion
-	Glu 131	deletion
-	Val 132	deletion
-	Ser 133	deletion
Thr 125	-	insertion
Thr 126	-	insertion
Thr 127	-	insertion
Ser 233	Lys 239	engineered mutation
Ser 234	Glu 240	engineered mutation
Ser 248	Lys 254	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51211.1
Non-Polymers*	Number of molecules	2
	Total formula weight	212.2
All*	Total formula weight	51423.3

*Water molecules are not included.