7FDD
A Crystal structure of OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.691, 128.806, 65.777 |
| Unit cell angles | 90.00, 118.87, 90.00 |
Refinement procedure
| Resolution | 19.490 - 2.900 |
| R-factor | 0.271 |
| Rwork | 0.270 |
| R-free | 0.29970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ljy |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.509 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.490 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.073 | 0.459 |
| Number of reflections | 19789 | 5091 |
| <I/σ(I)> | 11.5 | 2.4 |
| Completeness [%] | 93.4 | 84.4 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | Protein (16.0 mg/mL) 36% Polyethylene glycol 400, 0.1 M Imidazole (pH7.0) |
