7FDD
A Crystal structure of OspA mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.691, 128.806, 65.777 |
Unit cell angles | 90.00, 118.87, 90.00 |
Refinement procedure
Resolution | 19.490 - 2.900 |
R-factor | 0.271 |
Rwork | 0.270 |
R-free | 0.29970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ljy |
RMSD bond length | 0.002 |
RMSD bond angle | 0.509 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.490 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.073 | 0.459 |
Number of reflections | 19789 | 5091 |
<I/σ(I)> | 11.5 | 2.4 |
Completeness [%] | 93.4 | 84.4 |
Redundancy | 3.1 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | Protein (16.0 mg/mL) 36% Polyethylene glycol 400, 0.1 M Imidazole (pH7.0) |