7F8V

Cryo-EM structure of the cholecystokinin receptor CCKBR in complex with gastrin-17 and Gi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-2	polymer	355	40502.9	1	UniProt (P04899) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
4	E	Gastrin-17	polymer	17	2098.2	1	Pfam (PF00918) UniProt (by SIFTS) (P01350) In PDB	Homo sapiens
5	R	Gastrin/cholecystokinin type B receptor	polymer	465	50524.9	1	UniProt (P32239) Pfam (PF00001) In PDB	Homo sapiens (Human)	CCK-B receptor,CCK-BR,Cholecystokinin-2 receptor,CCK2-R

Sequence modifications

A: 1 - 355 (UniProt: P04899)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 204	Gly 204	engineered mutation
Ala 246	Glu 246	engineered mutation
Ser 327	Ala 327	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 2 - 418 (UniProt: P32239)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Pro 1	-	expression tag
Glu 419	-	expression tag
Phe 420	-	expression tag
Leu 421	-	expression tag
Glu 422	-	expression tag
Val 423	-	expression tag
Leu 424	-	expression tag
Phe 425	-	expression tag
Gln 426	-	expression tag
Gly 427	-	expression tag
Pro 428	-	expression tag
Trp 429	-	expression tag
Ser 430	-	expression tag
His 431	-	expression tag
Pro 432	-	expression tag
Gln 433	-	expression tag
Phe 434	-	expression tag
Glu 435	-	expression tag
Lys 436	-	expression tag
Gly 437	-	expression tag
Gly 438	-	expression tag
Gly 439	-	expression tag
Ser 440	-	expression tag
Gly 441	-	expression tag
Gly 442	-	expression tag
Gly 443	-	expression tag
Ser 444	-	expression tag
Gly 445	-	expression tag
Gly 446	-	expression tag
Ser 447	-	expression tag
Ala 448	-	expression tag
Trp 449	-	expression tag
Ser 450	-	expression tag
His 451	-	expression tag
Pro 452	-	expression tag
Gln 453	-	expression tag
Phe 454	-	expression tag
Glu 455	-	expression tag
Lys 456	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	139731.5
All*	Total formula weight	139731.5

*Water molecules are not included.