7F6H

Cryo-EM structure of human bradykinin receptor BK2R in complex Gq proteins and bradykinin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Bradykinin receptor BK2R	polymer	770	86464.7	1	Pfam (PF00001) UniProt (by SIFTS) (P30411)	Homo sapiens
2	B (L)	Bradykinin	polymer	9	1062.2	1	UniProt (by SIFTS) (P01042)	Homo sapiens
3	C (B)	Guanine nucleotide-binding protein G(q) subunit alpha	polymer	369	43534.4	1	UniProt (P50148) Pfam (PF00503)	Homo sapiens (Human)	Guanine nucleotide-binding protein alpha-q
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	354	39086.6	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
5	E (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	81	9242.6	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
6	F, G (A)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)

Sequence modifications

B: 2 - 359 (UniProt: P50148)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gln 183	Arg 183	variant
Leu 209	Gln 209	variant

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -13	-	initiating methionine
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

D: 2 - 71 (UniProt: P59768)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	179390.7
Non-Polymers*	Number of molecules	2
	Total formula weight	773.3
All*	Total formula weight	180164.0

*Water molecules are not included.