7F6G

Cryo-EM structure of human angiotensin receptor AT1R in complex Gq proteins and Sar1-AngII

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Angiotensin receptor AT1R	polymer	725	81574.4	1		Homo sapiens
2	L	SAR1-AngII	polymer	8	1004.2	1		Homo sapiens
3	B	Guanine nucleotide-binding protein G(q) subunit alpha	polymer	369	43534.4	1	UniProt (P50148) Pfam (PF00503) In PDB	Homo sapiens (Human)	Guanine nucleotide-binding protein alpha-q
4	C	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	354	39086.6	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
5	D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	81	9242.6	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
6	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
7	A	CHOLESTEROL	non-polymer		386.7	6	Chemie (CLR)

Sequence modifications

B: 2 - 359 (UniProt: P50148)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gln 183	Arg 183	conflict
Leu 209	Gln 209	conflict

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -13	-	initiating methionine
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

D: 2 - 71 (UniProt: P59768)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	174442.3
Non-Polymers*	Number of molecules	7
	Total formula weight	2541.1
All*	Total formula weight	176983.4

*Water molecules are not included.