7F4Y

Crystal structure of replisomal dimer of DNA polymerase from bacteriophage RB69 with DNA duplexes

Replaces:  5GNQ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	DNA polymerase	polymer	908	105058.6	2	UniProt (Q38087) Pfam (PF03104) Pfam (PF00136)	Enterobacteria phage RB69	Gp43
2	B, E (T, S)	DNA (5'-D(*TP*CP*AP*AP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*TP*C)-3')	polymer	19	5789.8	2		synthetic construct
3	C, F (P, Q)	DNA (5'-D(*GP*AP*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*C)-3')	polymer	15	4610.0	2		synthetic construct
4	G (A)	2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DUP)
5	H, J (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6	I, K (A, B)	GUANOSINE-5'-MONOPHOSPHATE	non-polymer		363.2	2	Chemie (5GP)
7	L (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8	M, N, O, P, Q... (A, T, P, B, S...)	water	water		18.0	1932	Chemie (HOH)

Sequence modifications

A, B: 1 - 903 (UniProt: Q38087)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 222	Asp 222	engineered mutation
Ala 327	Asp 327	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	230916.7
Non-Polymers*	Number of molecules	6
	Total formula weight	1298.1
All*	Total formula weight	232214.8

*Water molecules are not included.