7F4Y
Crystal structure of replisomal dimer of DNA polymerase from bacteriophage RB69 with DNA duplexes
Replaces: 5GNQExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-02 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 164.440, 164.440, 165.601 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 2.200 |
| R-factor | 0.19615 |
| Rwork | 0.194 |
| R-free | 0.24548 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nci |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.410 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.130 | |
| Number of reflections | 130936 | 9132 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.4 | 12 |
| CC(1/2) | 0.990 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | sodium acetate, isopropanol, magnesium chloride, sodium chloride, spermine tetrahydrochloride |
