7F1R

Cryo-EM structure of the chemokine receptor CCR5 in complex with RANTES and Gi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	C-C motif chemokine 5,C-C chemokine receptor type 5	polymer	462	52101.2	1	UniProt (P13501) UniProt (P51681) Pfam (PF00048) Pfam (PF00001)	Homo sapiens (Human)	EoCP,Eosinophil chemotactic cytokine,SIS-delta,Small-inducible cytokine A5,T cell-specific protein P228,TCP228,T-cell-specific protein RANTES,C-C CKR-5,CC-CKR-5,CCR-5,CCR5,CHEMR13,HIV-1 fusion coreceptor
2	B (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (Human)	Transducin beta chain 1
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I

Sequence modifications

R: 1 - 68 (UniProt: P13501)

PDB	External Database	Details
Cys 28	Phe 51	engineered mutation
Glu 69	-	linker
Phe 70	-	linker
Leu 71	-	linker
Glu 72	-	linker
Gly 73	-	linker
Ser 74	-	linker
Gly 75	-	linker
Ser 76	-	linker
Gly 77	-	linker
Ser 78	-	linker
Gly 79	-	linker
Ser 80	-	linker
Gly 81	-	linker
Ser 82	-	linker
Gly 83	-	linker
Ser 84	-	linker
Gly 85	-	linker
Ser 86	-	linker
Gly 87	-	linker
Ser 88	-	linker
Gly 89	-	linker
Ser 90	-	linker
Gly 91	-	linker
Ser 92	-	linker
Gly 93	-	linker
Ser 94	-	linker
Gly 95	-	linker
Ser 96	-	linker

R: 97 - 414 (UniProt: P51681)

PDB	External Database	Details
Asn 258	Gly 163	engineered mutation
Cys 267	Glu 172	engineered mutation
Glu 415	-	expression tag
Phe 416	-	expression tag
Leu 417	-	expression tag
Glu 418	-	expression tag
Val 419	-	expression tag
Leu 420	-	expression tag
Phe 421	-	expression tag
Gln 422	-	expression tag
Gly 423	-	expression tag
Pro 424	-	expression tag
Gly 425	-	expression tag
Ser 426	-	expression tag
Trp 427	-	expression tag
Ser 428	-	expression tag
His 429	-	expression tag
Pro 430	-	expression tag
Gln 431	-	expression tag
Phe 432	-	expression tag
Glu 433	-	expression tag
Lys 434	-	expression tag
Gly 435	-	expression tag
Ser 436	-	expression tag
Gly 437	-	expression tag
Ala 438	-	expression tag
Gly 439	-	expression tag
Ala 440	-	expression tag
Ser 441	-	expression tag
Ala 442	-	expression tag
Gly 443	-	expression tag
Ser 444	-	expression tag
Trp 445	-	expression tag
Ser 446	-	expression tag
His 447	-	expression tag
Pro 448	-	expression tag
Gln 449	-	expression tag
Phe 450	-	expression tag
Glu 451	-	expression tag
Lys 452	-	expression tag
Gly 453	-	expression tag
Ser 454	-	expression tag
Asp 455	-	expression tag
Tyr 456	-	expression tag
Lys 457	-	expression tag
Asp 458	-	expression tag
Asp 459	-	expression tag
Asp 460	-	expression tag
Asp 461	-	expression tag
Lys 462	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	137826.4
All*	Total formula weight	137826.4

*Water molecules are not included.