7F1Q

Cryo-EM structure of the chemokine receptor CCR5 in complex with MIP-1a and Gi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	C-C motif chemokine 3,C-C chemokine receptor type 5	polymer	457	51241.0	1	UniProt (P10147) UniProt (P51681) Pfam (PF00048) Pfam (PF00001) In PDB	Homo sapiens (Human)	G0/G1 switch regulatory protein 19-1,Macrophage inflammatory protein 1-alpha,MIP-1-alpha,PAT 464.1,SIS-beta,Small-inducible cytokine A3,Tonsillar lymphocyte LD78 alpha protein,C-C CKR-5,CC-CKR-5,CCR-5,CCR5,CHEMR13,HIV-1 fusion coreceptor
2	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
4	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I

Sequence modifications

R: 1 - 69 (UniProt: P10147)

PDB	External Database	Details
Cys 15	Thr 38	engineered mutation
Gly 74	-	linker
Ser 75	-	linker
Gly 76	-	linker
Ser 77	-	linker
Gly 78	-	linker
Ser 79	-	linker
Gly 80	-	linker
Ser 81	-	linker
Gly 82	-	linker
Ser 83	-	linker
Gly 84	-	linker
Ser 85	-	linker
Gly 86	-	linker
Ser 87	-	linker
Gly 88	-	linker
Ser 89	-	linker
Gly 90	-	linker
Ser 91	-	linker
Gly 92	-	linker
Ser 93	-	linker
Gly 94	-	linker
Ser 95	-	linker
Gly 96	-	linker
Ser 97	-	linker

R: 98 - 415 (UniProt: P51681)

PDB	External Database	Details
Cys 112	Thr 16	engineered mutation
Asn 259	Gly 163	engineered mutation
Leu 416	-	expression tag
Glu 417	-	expression tag
Val 418	-	expression tag
Leu 419	-	expression tag
Phe 420	-	expression tag
Gln 421	-	expression tag
Gly 422	-	expression tag
Pro 423	-	expression tag
Gly 424	-	expression tag
Ser 425	-	expression tag
Trp 426	-	expression tag
Ser 427	-	expression tag
His 428	-	expression tag
Pro 429	-	expression tag
Gln 430	-	expression tag
Phe 431	-	expression tag
Glu 432	-	expression tag
Lys 433	-	expression tag
Gly 434	-	expression tag
Ser 435	-	expression tag
Gly 436	-	expression tag
Ala 437	-	expression tag
Gly 438	-	expression tag
Ala 439	-	expression tag
Ser 440	-	expression tag
Ala 441	-	expression tag
Gly 442	-	expression tag
Ser 443	-	expression tag
Trp 444	-	expression tag
Ser 445	-	expression tag
His 446	-	expression tag
Pro 447	-	expression tag
Gln 448	-	expression tag
Phe 449	-	expression tag
Glu 450	-	expression tag
Lys 451	-	expression tag
Gly 452	-	expression tag
Ser 453	-	expression tag
Asp 454	-	expression tag
Tyr 455	-	expression tag
Lys 456	-	expression tag
Asp 457	-	expression tag
Asp 458	-	expression tag
Asp 459	-	expression tag
Asp 460	-	expression tag
Lys 461	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	136966.3
All*	Total formula weight	136966.3

*Water molecules are not included.