7F1O

Cryo-EM structure of the GDP-bound dopamine receptor 1 and mini-Gs complex with Nb35

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	F	D(1A) dopamine receptor	polymer	473	52409.7	1	UniProt (P21728) Pfam (PF00001) In PDB	Homo sapiens (Human)	Dopamine D1 receptor
2	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	248	28907.7	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	357	39286.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
4	D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
5	E	Nanobody 35	polymer	160	17352.5	1		synthetic construct
6	F	L-DOPAMINE	non-polymer		153.2	1	Chemie (LDP)
7	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8	A	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)

Sequence modifications

F: 1 - 446 (UniProt: P21728)

PDB	External Database	Details
Met -26	-	initiating methionine
Lys -25	-	expression tag
Thr -24	-	expression tag
Ile -23	-	expression tag
Ile -22	-	expression tag
Ala -21	-	expression tag
Leu -20	-	expression tag
Ser -19	-	expression tag
Tyr -18	-	expression tag
Ile -17	-	expression tag
Phe -16	-	expression tag
Cys -15	-	expression tag
Leu -14	-	expression tag
Val -13	-	expression tag
Phe -12	-	expression tag
Ala -11	-	expression tag
Asp -10	-	expression tag
Tyr -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Ile -1	-	expression tag
Asp 0	-	expression tag

A: 6 - 64 (UniProt: P63092)

PDB	External Database	Details
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation

A: 204 - 394 (UniProt: P63092)

PDB	External Database	Details
Gly 65	-	linker
Gly 66	-	linker
Ser 67	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 235	engineered mutation
Asp 252	Ser 238	engineered mutation
-	Met 241	deletion
-	Val 242	deletion
-	Ile 243	deletion
-	Arg 244	deletion
-	Glu 245	deletion
-	Asp 246	deletion
-	Asn 247	deletion
-	Gln 248	deletion
-	Thr 249	deletion
-	Asn 250	deletion
Ala 372	Ile 358	engineered mutation
Ile 375	Val 361	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

D: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	145801.8
Non-Polymers*	Number of molecules	3
	Total formula weight	620.7
All*	Total formula weight	146422.5

*Water molecules are not included.