7EZM

Cryo-EM structure of an activated Cholecystokinin A receptor (CCKAR)-Gq complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	fusion protein of Guanine nucleotide-binding protein G(i) subunit alpha-1 and Guanine nucleotide-binding protein G(q) subunit alpha-q	polymer	353	41268.9	1	UniProt (P63096) UniProt (P50148) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein,Guanine nucleotide-binding protein alpha-q
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (D)	Cholecystokinin receptor type A	polymer	428	47886.1	1	UniProt (P32238) Pfam (PF00001) Pfam (PF09193)	Homo sapiens (Human)	CCK-A receptor,CCK-AR,Cholecystokinin-1 receptor,CCK1-R
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
5	E (H)	scFv16	polymer	247	26277.3	1		synthetic construct
6	F (P)	Cholecystokinin-8	polymer	9	1291.4	1	UniProt (by SIFTS) (P06307)	Homo sapiens (Human)

Sequence modifications

A: 1 - 36 (UniProt: P63096)
A: 37 - 359 (UniProt: P50148)

PDB	External Database	Details
Ala 208	Gly 208	engineered mutation
Ser 331	Ala 331	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	163329.2
All*	Total formula weight	163329.2

*Water molecules are not included.