7EZK
Cryo-EM structure of an activated Cholecystokinin A receptor (CCKAR)-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Chimera of Guanine nucleotide-binding protein G(i) subunit alpha-1 and Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 361 | 41879.5 | 1 | UniProt (P63096) UniProt (P63092) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein,Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-inhibiting G alpha protein,Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (Human) | Transducin beta chain 1 |
| 3 | C (D) | Cholecystokinin receptor type A | polymer | 428 | 47886.1 | 1 | UniProt (P32238) Pfam (PF09193) Pfam (PF00001) | Homo sapiens (Human) | CCK-A receptor,CCK-AR,Cholecystokinin-1 receptor,CCK1-R |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 5 | E (P) | Cholecystokinin-8 | polymer | 9 | 1291.4 | 1 | UniProt (by SIFTS) (P06307) | Homo sapiens (Human) |
Sequence modifications
A: 1 - 18 (UniProt: P63096)
A: 19 - 59 (UniProt: P63092)
A: 60 - 180 (UniProt: P63096)
A: 181 - 361 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
A: 19 - 59 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Asp 42 | Gly 49 | engineered mutation |
| Asn 43 | Glu 50 | engineered mutation |
| Tyr 56 | Leu 63 | engineered mutation |
A: 181 - 361 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Ala 203 | Gly 226 | engineered mutation |
| Asp 226 | Ala 249 | engineered mutation |
| Asp 229 | Ser 252 | engineered mutation |
| - | Asn 254 | deletion |
| - | Met 255 | deletion |
| - | Val 256 | deletion |
| - | Ile 257 | deletion |
| - | Arg 258 | deletion |
| - | Glu 259 | deletion |
| - | Asp 260 | deletion |
| - | Asn 261 | deletion |
| - | Gln 262 | deletion |
| - | Thr 263 | deletion |
| Asp 239 | Leu 272 | engineered mutation |
| Ser 333 | Ala 366 | engineered mutation |
| Ala 339 | Ile 372 | engineered mutation |
| Ile 342 | Val 375 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 137662.5 | |
| All* | Total formula weight | 137662.5 |
