7EXU

GH127 beta-L-arabinofuranosidase HypBA1 E322Q mutant complexed with p-nitrophenyl beta-L-arabinofuranoside

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Non-reducing end beta-L-arabinofuranosidase	polymer	669	74456.9	1	UniProt (E8MGH8) Pfam (PF07944) Pfam (PF20736) Pfam (PF20737) In PDB	Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)	Beta-L-arabinofuranosidase HypBA1
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	A	(2S,3R,4R,5R)-2-(hydroxymethyl)-5-(4-nitrophenoxy)oxolane-3,4-diol	non-polymer		271.2	1	Chemie (07Y)
4		water	water		18.0	25	Chemie (HOH)

Sequence modifications

A: 1 - 658 (UniProt: E8MGH8)

PDB	External Database	Details
Gln 322	Glu 322	engineered mutation
Ala 659	-	expression tag
Ala 660	-	expression tag
Ala 661	-	expression tag
Leu 662	-	expression tag
Glu 663	-	expression tag
His 664	-	expression tag
His 665	-	expression tag
His 666	-	expression tag
His 667	-	expression tag
His 668	-	expression tag
His 669	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	74456.9
Non-Polymers*	Number of molecules	2
	Total formula weight	336.6
All*	Total formula weight	74793.6

*Water molecules are not included.