7EXU

GH127 beta-L-arabinofuranosidase HypBA1 E322Q mutant complexed with p-nitrophenyl beta-L-arabinofuranoside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-27
Detector	ADSC QUANTUM 270
Wavelength(s)	1.1000
Spacegroup name	P 32 2 1
Unit cell lengths	78.182, 78.182, 253.502
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.310 - 2.300
R-factor	0.2536
Rwork	0.252
R-free	0.28980
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3wkx
RMSD bond length	0.005
RMSD bond angle	1.412
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.310	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.113	3.663
Rpim	0.036	1.144
Number of reflections	41037	3910
<I/σ(I)>	14.5	1
Completeness [%]	100.0	100
Redundancy	10.9	11.1
CC(1/2)	0.999	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	0.7 M sodium citrate, 0.1 M MES-NaOH (pH 6.5), 10 mM DTT