7EWX

Crystal structure of Ebinur Lake virus cap snatching endonuclease (P78A mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Replicase	polymer	219	25155.8	2	UniProt (A0A059WLS9) Pfam (PF15518) In PDB	Abbey lake orthobunyavirus	Transcriptase,L protein
2	A, B	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	A, B	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
4		water	water		18.0	216	Chemie (HOH)

Sequence modifications

A, B: 1 - 183 (UniProt: A0A059WLS9)

PDB	External Database	Details
Met -35	-	initiating methionine
Gly -34	-	expression tag
Ser -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Leu -22	-	expression tag
Val -21	-	expression tag
Pro -20	-	expression tag
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Met -15	-	expression tag
Ala -14	-	expression tag
Ser -13	-	expression tag
Met -12	-	expression tag
Thr -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Gln -8	-	expression tag
Gln -7	-	expression tag
Met -6	-	expression tag
Gly -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Ala 78	Pro 78	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	50311.7
Non-Polymers*	Number of molecules	5
	Total formula weight	216.2
All*	Total formula weight	50527.9

*Water molecules are not included.