7EWX
Crystal structure of Ebinur Lake virus cap snatching endonuclease (P78A mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.319, 41.399, 85.776 |
Unit cell angles | 90.00, 104.94, 90.00 |
Refinement procedure
Resolution | 28.590 - 1.950 |
R-factor | 0.2033 |
Rwork | 0.201 |
R-free | 0.24290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xi5 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
Rmerge | 0.071 | 0.066 | 0.345 |
Rmeas | 0.085 | 0.080 | 0.416 |
Rpim | 0.045 | 0.043 | 0.227 |
Total number of observations | 104698 | ||
Number of reflections | 33813 | 3198 | 3458 |
<I/σ(I)> | 10.1 | ||
Completeness [%] | 96.3 | 87.4 | 99.1 |
Redundancy | 3.1 | 2.9 | 3 |
CC(1/2) | 0.990 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.7 | 289 | PEG 3350 |