7EWW

Crystal structure of Ebinur Lake virus cap snatching endonuclease (N52A mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Replicase	polymer	219	25138.8	2	UniProt (A0A059WLS9) Pfam (PF15518)	Abbey lake orthobunyavirus	Transcriptase,L protein
2	C, D (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	E, F (A, B)	water	water		18.0	51	Chemie (HOH)

Sequence modifications

A, B: 1 - 183 (UniProt: A0A059WLS9)

PDB	External Database	Details
Met -35	-	initiating methionine
Gly -34	-	expression tag
Ser -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Leu -22	-	expression tag
Val -21	-	expression tag
Pro -20	-	expression tag
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
Met -15	-	expression tag
Ala -14	-	expression tag
Ser -13	-	expression tag
Met -12	-	expression tag
Thr -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Gln -8	-	expression tag
Gln -7	-	expression tag
Met -6	-	expression tag
Gly -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Ala 52	Asn 52	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	50277.7
Non-Polymers*	Number of molecules	2
	Total formula weight	109.9
All*	Total formula weight	50387.6

*Water molecules are not included.