7EW4

Cryo-EM structure of CYM-5541-bound Sphingosine 1-phosphate receptor 3 in complex with Gi protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	356	39141.8	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
4	R	Sphingosine 1-phosphate receptor 3	polymer	412	46435.9	1	UniProt (Q99500) Pfam (PF00001) In PDB	Homo sapiens (Human)	S1P receptor 3,S1P3,Endothelial differentiation G-protein coupled receptor 3,Sphingosine 1-phosphate receptor Edg-3,S1P receptor Edg-3
5	S	scFV16	polymer	266	28636.8	1		Homo sapiens (Human)
6	R	N,N-dicyclohexyl-5-cyclopropyl-1,2-oxazole-3-carboxamide	non-polymer		316.4	1	Chemie (JF9)
7	R	PALMITIC ACID	non-polymer		256.4	2	Chemie (PLM)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -15	-	initiating methionine
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 1 - 378 (UniProt: Q99500)

PDB	External Database	Details
Met -33	-	initiating methionine
Lys -32	-	expression tag
Thr -31	-	expression tag
Ile -30	-	expression tag
Ile -29	-	expression tag
Ala -28	-	expression tag
Leu -27	-	expression tag
Ser -26	-	expression tag
Tyr -25	-	expression tag
Ile -24	-	expression tag
Phe -23	-	expression tag
Cys -22	-	expression tag
Leu -21	-	expression tag
Val -20	-	expression tag
Phe -19	-	expression tag
Ala -18	-	expression tag
Asp -17	-	expression tag
Tyr -16	-	expression tag
Lys -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Ala -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	162490.7
Non-Polymers*	Number of molecules	3
	Total formula weight	829.3
All*	Total formula weight	163320.0

*Water molecules are not included.