7EW1

Cryo-EM structure of siponimod -bound Sphingosine-1-phosphate receptor 5 in complex with Gi protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	356	39141.8	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
3	C (D)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
4	D (E)	scFv16	polymer	266	28636.8	1		Homo sapiens
5	E (A)	Sphingosine 1-phosphate receptor 5	polymer	394	42630.2	1	UniProt (Q9H228) Pfam (PF00001)	Homo sapiens (Human)	S1P receptor 5,S1P5,Endothelial differentiation G-protein-coupled receptor 8,Sphingosine 1-phosphate receptor Edg-8,S1P receptor Edg-8
6	F (A)	1-[[4-[(~{E})-~{N}-[[4-cyclohexyl-3-(trifluoromethyl)phenyl]methoxy]-~{C}-methyl-carbonimidoyl]-2-ethyl-phenyl]methyl]azetidine-3-carboxylic acid	non-polymer		516.6	1	Chemie (J8C)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -15	-	initiating methionine
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

A: 1 - 344 (UniProt: Q9H228)

PDB	External Database	Details
Met -32	-	initiating methionine
Lys -31	-	expression tag
Thr -30	-	expression tag
Ile -29	-	expression tag
Ile -28	-	expression tag
Ala -27	-	expression tag
Leu -26	-	expression tag
Ser -25	-	expression tag
Tyr -24	-	expression tag
Ile -23	-	expression tag
Phe -22	-	expression tag
Cys -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Phe -18	-	expression tag
Ala -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Lys -9	-	expression tag
Met -8	-	expression tag
Gly -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Glu 345	-	expression tag
Asn 346	-	expression tag
Leu 347	-	expression tag
Tyr 348	-	expression tag
Phe 349	-	expression tag
Gln 350	-	expression tag
Gly 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag
His 357	-	expression tag
His 358	-	expression tag
His 359	-	expression tag
His 360	-	expression tag
His 361	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	158685.0
Non-Polymers*	Number of molecules	1
	Total formula weight	516.6
All*	Total formula weight	159201.6

*Water molecules are not included.