7EW1
Cryo-EM structure of siponimod -bound Sphingosine-1-phosphate receptor 5 in complex with Gi protein
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 356 | 39141.8 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (Human) | Transducin beta chain 1 |
2 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (Human) | G gamma-I |
3 | D | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40415.0 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
4 | E | scFv16 | polymer | 266 | 28636.8 | 1 | Homo sapiens | ||
5 | A | Sphingosine 1-phosphate receptor 5 | polymer | 394 | 42630.2 | 1 | UniProt (Q9H228) Pfam (PF00001) In PDB | Homo sapiens (Human) | S1P receptor 5,S1P5,Endothelial differentiation G-protein-coupled receptor 8,Sphingosine 1-phosphate receptor Edg-8,S1P receptor Edg-8 |
6 | A | 1-[[4-[(~{E})-~{N}-[[4-cyclohexyl-3-(trifluoromethyl)phenyl]methoxy]-~{C}-methyl-carbonimidoyl]-2-ethyl-phenyl]methyl]azetidine-3-carboxylic acid | non-polymer | 516.6 | 1 | Chemie (J8C) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
A: 1 - 344 (UniProt: Q9H228)
PDB | External Database | Details |
---|---|---|
Met -15 | - | initiating methionine |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
Leu -10 | - | expression tag |
Glu -9 | - | expression tag |
Val -8 | - | expression tag |
Leu -7 | - | expression tag |
Phe -6 | - | expression tag |
Gln -5 | - | expression tag |
Gly -4 | - | expression tag |
Pro -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 1 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met -32 | - | initiating methionine |
Lys -31 | - | expression tag |
Thr -30 | - | expression tag |
Ile -29 | - | expression tag |
Ile -28 | - | expression tag |
Ala -27 | - | expression tag |
Leu -26 | - | expression tag |
Ser -25 | - | expression tag |
Tyr -24 | - | expression tag |
Ile -23 | - | expression tag |
Phe -22 | - | expression tag |
Cys -21 | - | expression tag |
Leu -20 | - | expression tag |
Val -19 | - | expression tag |
Phe -18 | - | expression tag |
Ala -17 | - | expression tag |
Asp -16 | - | expression tag |
Tyr -15 | - | expression tag |
Lys -14 | - | expression tag |
Asp -13 | - | expression tag |
Asp -12 | - | expression tag |
Asp -11 | - | expression tag |
Asp -10 | - | expression tag |
Lys -9 | - | expression tag |
Met -8 | - | expression tag |
Gly -7 | - | expression tag |
Glu -6 | - | expression tag |
Asn -5 | - | expression tag |
Leu -4 | - | expression tag |
Tyr -3 | - | expression tag |
Phe -2 | - | expression tag |
Gln -1 | - | expression tag |
Gly 0 | - | expression tag |
Glu 345 | - | expression tag |
Asn 346 | - | expression tag |
Leu 347 | - | expression tag |
Tyr 348 | - | expression tag |
Phe 349 | - | expression tag |
Gln 350 | - | expression tag |
Gly 351 | - | expression tag |
His 352 | - | expression tag |
His 353 | - | expression tag |
His 354 | - | expression tag |
His 355 | - | expression tag |
His 356 | - | expression tag |
His 357 | - | expression tag |
His 358 | - | expression tag |
His 359 | - | expression tag |
His 360 | - | expression tag |
His 361 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 158685.0 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 516.6 | |
All* | Total formula weight | 159201.6 |