7EU0
The cryo-EM structure of A. thaliana Pol IV-RDR2 backtracked complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase IV subunit 1 | polymer | 1510 | 169535.3 | 1 | Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF11523) UniProt (by SIFTS) (Q9LQ02) | Arabidopsis thaliana (Mouse-ear cress) | |
| 2 | B (B) | DNA-directed RNA polymerases IV and V subunit 2 | polymer | 1172 | 132848.0 | 1 | UniProt (Q9LK40) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF00562) | Arabidopsis thaliana (thale cress) | DNA-directed RNA polymerase D subunit 2a,AtNRPD2a,Nuclear RNA polymerase D 2a,Nuclear RNA polymerase E 2,Protein DEFECTIVE IN MERISTEM SILENCING 2,Protein DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 2,RNA polymerase IV subunit 2a,POL IV 2a |
| 3 | C (C) | DNA-directed RNA polymerases II, IV and V subunit 3 | polymer | 319 | 35503.2 | 1 | UniProt (Q39211) Pfam (PF01193) Pfam (PF01000) | Arabidopsis thaliana (thale cress) | DNA-directed RNA polymerase II 36 kDa polypeptide A,DNA-directed RNA polymerase II subunit RPB3-A,RNA polymerase II subunit 3-A,RNA polymerase II subunit B3-A |
| 4 | D (E) | DNA-directed RNA polymerases II and IV subunit 5A | polymer | 205 | 24340.4 | 1 | UniProt (O81098) Pfam (PF03871) Pfam (PF01191) | Arabidopsis thaliana (thale cress) | RPB5a,DNA-directed RNA polymerase II subunit E,RNA polymerase I,II and III 24.3 kDa subunit,AtRPB24.3 |
| 5 | E (F) | DNA-directed RNA polymerases II, IV and V subunit 6A | polymer | 144 | 16670.3 | 1 | UniProt (Q9FJ98) Pfam (PF01192) | Arabidopsis thaliana (thale cress) | RNA polymerase Rpb6 |
| 6 | F (H) | DNA-directed RNA polymerases II, IV and V subunit 8B | polymer | 146 | 16626.3 | 1 | UniProt (Q9M1A8) Pfam (PF03870) | Arabidopsis thaliana (thale cress) | RNA polymerase Rpb8 |
| 7 | G (I) | DNA-directed RNA polymerases II, IV and V subunit 9A | polymer | 114 | 13297.1 | 1 | UniProt (Q6NLH0) Pfam (PF02150) Pfam (PF01096) | Arabidopsis thaliana (thale cress) | |
| 8 | H (J) | DNA-directed RNA polymerases II, IV and V subunit 10 | polymer | 71 | 8323.7 | 1 | UniProt (Q8LFJ6) Pfam (PF01194) | Arabidopsis thaliana (thale cress) | DNA-directed RNA Polymerase II subunit L |
| 9 | I (K) | DNA-directed RNA polymerases II, IV and V subunit 11 | polymer | 116 | 13582.3 | 1 | UniProt (Q38859) Pfam (PF13656) | Arabidopsis thaliana (thale cress) | DNA-directed RNA polymerase II 13.6 kDa polypeptide,DNA-directed RNA polymerase II subunit J,DNA-directed RNA polymerase II subunit RPB11,RNA polymerase II subunit B11 |
| 10 | J (L) | DNA-directed RNA polymerases II, IV and V subunit 12 | polymer | 51 | 5905.8 | 1 | UniProt (Q9FLM8) Pfam (PF03604) | Arabidopsis thaliana (thale cress) | DNA-directed RNA Polymerase II subunit K |
| 11 | K (M) | RNA-dependent RNA polymerase 2 | polymer | 1133 | 129494.5 | 1 | UniProt (O82504) Pfam (PF05183) | Arabidopsis thaliana (thale cress) | AtRDRP2,Protein SILENCING MOVEMENT DEFICIENT 1,RNA-directed RNA polymerase 2 |
| 12 | L (N) | DNA (5'-D(*CP*TP*GP*TP*GP*CP*TP*TP*AP*TP*CP*GP*GP*TP*AP*GP*AP*GP*TP*G)-3') | polymer | 20 | 6196.0 | 1 | synthetic construct | ||
| 13 | M (O) | RNA (39-MER) | polymer | 39 | 12215.0 | 1 | synthetic construct | ||
| 14 | N (Q) | DNA (33-MER) | polymer | 33 | 9954.4 | 1 | synthetic construct | ||
| 15 | O (R) | RNA (5'-R(*CP*CP*GP*A)-3') | polymer | 4 | 1239.8 | 1 | synthetic construct | ||
| 16 | P, R, S, T, U... (A, B, C, I, J...) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 17 | Q, Y (A, M) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 595732.3 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 571.9 | |
| All* | Total formula weight | 596304.1 |
