7EPT

Structural basis for the tethered peptide activation of adhesion GPCRs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45727.4	1	Pfam (PF00503) UniProt (by SIFTS) (P63092)	Homo sapiens (Human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39489.2	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody-35	polymer	128	13885.4	1		synthetic construct
5	E (R)	Adhesion G-protein coupled receptor D1	polymer	283	31096.7	1	UniProt (Q6QNK2) Pfam (PF00002)	Homo sapiens (human)	G-protein coupled receptor 133,G-protein coupled receptor PGR25
6	F (R)	PALMITIC ACID	non-polymer		256.4	1	Chemie (PLM)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	138059.9
Non-Polymers*	Number of molecules	1
	Total formula weight	256.4
All*	Total formula weight	138316.4

*Water molecules are not included.