7EKH

Structure of SARS-CoV-2 spike receptor-binding domain Y453F mutation complexed with human ACE2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Angiotensin-converting enzyme 2	polymer	603	69982.6	1	UniProt (Q9BYF1) Pfam (PF01401)	Homo sapiens (Human)	ACE-related carboxypeptidase,Angiotensin-converting enzyme homolog,ACEH,Metalloprotease MPROT15
2	B (B)	Spike protein S1	polymer	229	25935.2	1	UniProt (P0DTC2) Pfam (PF09408)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)
3	C, D, E, G (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
4	F (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	H, I (A, B)	water	water		18.0	250	Chemie (HOH)

Sequence modifications

A: 19 - 615 (UniProt: Q9BYF1)

PDB	External Database	Details
His 616	-	expression tag
His 617	-	expression tag
His 618	-	expression tag
His 619	-	expression tag
His 620	-	expression tag
His 621	-	expression tag

B: 319 - 541 (UniProt: P0DTC2)

PDB	External Database	Details
Phe 453	Tyr 453	engineered mutation
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag
His 545	-	expression tag
His 546	-	expression tag
His 547	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	95917.8
Non-Polymers*	Number of molecules	5
	Total formula weight	950.2
All*	Total formula weight	96868.0

*Water molecules are not included.