7EJW

Crystal structure of FleN in complex with FleQ AAA+ doamain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Transcriptional antiactivator FleN	polymer	285	30484.3	2	UniProt (G3XD64) Pfam (PF10609)	Pseudomonas aeruginosa PAO1	Site-determining protein
2	C, D (C, D)	Transcriptional regulator FleQ	polymer	259	29098.5	2	UniProt (G3XCV0) Pfam (PF00158)	Pseudomonas aeruginosa PAO1
3	E, I (A, B)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	2	Chemie (AGS)
4	F, G, J, K (A, B, C)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	H (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	L, M, N, O (A, B, C, D)	water	water		18.0	1055	Chemie (HOH)

Sequence modifications

A, B: 1 - 280 (UniProt: G3XD64)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

C, D: 142 - 395 (UniProt: G3XCV0)

PDB	External Database	Details
Gly 137	-	expression tag
Pro 138	-	expression tag
Leu 139	-	expression tag
Gly 140	-	expression tag
Ser 141	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	119165.6
Non-Polymers*	Number of molecules	7
	Total formula weight	1235.8
All*	Total formula weight	120401.4

*Water molecules are not included.