7EGP

The structure of SWI/SNF-nucleosome complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	SWI/SNF chromatin-remodeling complex subunit SWI1	polymer	1093	124885.7	1	UniProt (P09547) Pfam (PF01388)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Regulatory protein GAM3,SWI/SNF complex subunit SWI1,Transcription regulatory protein ADR6,Transcription regulatory protein SWI1
2	B (C)	SWI/SNF chromatin-remodeling complex subunit SNF5	polymer	918	103954.4	1	UniProt (P18480) Pfam (PF04855)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	SWI/SNF complex subunit SNF5,Transcription factor TYE4,Transcription regulatory protein SNF5
3	C, G (D, E)	SWI/SNF complex subunit SWI3	polymer	836	94178.4	2	UniProt (P32591) Pfam (PF04433) Pfam (PF00249) Pfam (PF16495)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Transcription factor TYE2,Transcription regulatory protein SWI3
4	D (H)	Transcription regulatory protein SNF12	polymer	566	63947.6	1	UniProt (P53628)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	SWI/SNF complex component SWP73
5	E (I)	Transcription regulatory protein SNF6	polymer	332	37652.6	1	UniProt (P18888)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	SWI/SNF complex component SNF6
6	F (J)	SWI/SNF global transcription activator complex subunit SWP82	polymer	634	71510.8	1	UniProt (P43554) Pfam (PF08624)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
7	H (A)	Transcription regulatory protein SNF2	polymer	982	114311.9	1	UniProt (P22082) Pfam (PF00176) Pfam (PF00271) Pfam (PF14619)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	ATP-dependent helicase SNF2,Regulatory protein GAM1,Regulatory protein SWI2,SWI/SNF complex component SNF2,Transcription factor TYE3
8	I (L)	Regulator of Ty1 transposition protein 102	polymer	157	17817.6	1	UniProt (P53330) Pfam (PF09510)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
9	J (M)	Actin-related protein 7	polymer	477	53863.0	1	UniProt (Q12406) Pfam (PF00022)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Actin-like protein ARP7,Chromatin structure-remodeling complex protein ARP7,SWI/SNF complex component ARP7
10	K (N)	Actin-like protein ARP9	polymer	467	53131.9	1	UniProt (Q05123) Pfam (PF00022)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Chromatin structure-remodeling complex protein ARP9,SWI/SNF complex component ARP9
11	L, P (O, S)	Histone H3.2	polymer	135	15289.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
12	M, Q (P, T)	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
13	N, R (Q, U)	Histone H2A	polymer	129	13978.2	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
14	O, S (R, V)	Histone H2B 1.1	polymer	122	13524.8	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
15	T (W)	DNA (239-MER)	polymer	235	72380.1	1		synthetic construct
16	U (X)	DNA (239-MER)	polymer	235	72740.3	1		synthetic construct
17	V (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
18	W (A)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	1	Chemie (BEF)
19	X (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

B: 251 - 1314 (UniProt: P09547)

PDB	External Database	Details
Gly 1315	-	expression tag
Gly 1316	-	expression tag
Ser 1317	-	expression tag
Gly 1318	-	expression tag
Gly 1319	-	expression tag
Trp 1320	-	expression tag
Ser 1321	-	expression tag
His 1322	-	expression tag
Pro 1323	-	expression tag
Gln 1324	-	expression tag
Phe 1325	-	expression tag
Glu 1326	-	expression tag
Lys 1327	-	expression tag
Trp 1328	-	expression tag
Ser 1329	-	expression tag
His 1330	-	expression tag
Pro 1331	-	expression tag
Gln 1332	-	expression tag
Phe 1333	-	expression tag
Glu 1334	-	expression tag
Lys 1335	-	expression tag
Trp 1336	-	expression tag
Ser 1337	-	expression tag
His 1338	-	expression tag
Pro 1339	-	expression tag
Gln 1340	-	expression tag
Phe 1341	-	expression tag
Glu 1342	-	expression tag
Lys 1343	-	expression tag

C: 1 - 905 (UniProt: P18480)

PDB	External Database	Details
Gly 906	-	expression tag
Gly 907	-	expression tag
Ser 908	-	expression tag
Gly 909	-	expression tag
Gly 910	-	expression tag
Asp 911	-	expression tag
Tyr 912	-	expression tag
Lys 913	-	expression tag
Asp 914	-	expression tag
Asp 915	-	expression tag
Asp 916	-	expression tag
Asp 917	-	expression tag
Lys 918	-	expression tag

D, E: 1 - 825 (UniProt: P32591)

PDB	External Database	Details
Gly 826	-	expression tag
Gly 827	-	expression tag
Ser 828	-	expression tag
Gly 829	-	expression tag
Gly 830	-	expression tag
His 831	-	expression tag
His 832	-	expression tag
His 833	-	expression tag
His 834	-	expression tag
His 835	-	expression tag
His 836	-	expression tag

J: 1 - 623 (UniProt: P43554)

PDB	External Database	Details
Gly 624	-	expression tag
Gly 625	-	expression tag
Ser 626	-	expression tag
Gly 627	-	expression tag
Gly 628	-	expression tag
His 629	-	expression tag
His 630	-	expression tag
His 631	-	expression tag
His 632	-	expression tag
His 633	-	expression tag
His 634	-	expression tag

A: 430 - 1400 (UniProt: P22082)

PDB	External Database	Details
Gly 1401	-	expression tag
Gly 1402	-	expression tag
Ser 1403	-	expression tag
Gly 1404	-	expression tag
Gly 1405	-	expression tag
His 1406	-	expression tag
His 1407	-	expression tag
His 1408	-	expression tag
His 1409	-	expression tag
His 1410	-	expression tag
His 1411	-	expression tag

R, V: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	21
	Total formula weight	1082665.0
Non-Polymers*	Number of molecules	3
	Total formula weight	517.5
All*	Total formula weight	1083182.5

*Water molecules are not included.