7E8D

NSD2 E1099K mutant bound to nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3.1	polymer	135	15308.0	2	UniProt (P68431) Pfam (PF00125) In PDB	Homo sapiens (Human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
2	B, F	Histone H4	polymer	102	11263.2	2	UniProt (P62805) Pfam (PF15511) In PDB	Homo sapiens (Human)
3	C, G	Histone H2A type 1	polymer	129	13990.3	2	UniProt (P0C0S8) Pfam (PF00125) Pfam (PF16211) In PDB	Homo sapiens (Human)	H2A.1,Histone H2A/ptl
4	D, H	Histone H2B type 1-J	polymer	125	13804.0	2	UniProt (P06899) Pfam (PF00125) In PDB	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r
5	I	DNA (185-MER)	polymer	185	56841.2	1		synthetic construct
6	J	DNA (185-MER)	polymer	185	57392.5	1		synthetic construct
7	K	Histone-lysine N-methyltransferase NSD2	polymer	254	28986.1	1	UniProt (O96028) Pfam (PF17907) Pfam (PF00856) In PDB	Homo sapiens (Human)	Multiple myeloma SET domain-containing protein,MMSET,Nuclear SET domain-containing protein 2,Protein trithorax-5,Wolf-Hirschhorn syndrome candidate 1 protein
8	K	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
9	K	SINEFUNGIN	non-polymer		381.4	1	Chemie (SFG)

Sequence modifications

A, E: 1 - 135 (UniProt: P68431)

PDB	External Database	Details
Met 36	Lys 37	variant

K: 973 - 1226 (UniProt: O96028)

PDB	External Database	Details
Lys 1099	Glu 1099	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	251951.1
Non-Polymers*	Number of molecules	4
	Total formula weight	577.6
All*	Total formula weight	252528.7

*Water molecules are not included.