7E7N

Crystal structure of RSL mutant-R17A/R108A/R199A in complex with R3F

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Fucose-binding lectin protein,Fucose-binding lectin protein,Fucose-binding lectin protein	polymer	272	29091.6	1	UniProt (A0A0S4TLR1) Pfam (PF07938) UniProt (by SIFTS) (A0A0S4WQH1) In PDB	Ralstonia solanacearum (Pseudomonas solanacearum)	Putative fucose-binding lectin protein
2	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	A	2-[2-[2-[4-[[(2R,3S,4R,5S,6S)-6-methyl-3,4,5-tris(oxidanyl)oxan-2-yl]oxymethyl]-1,2,3-triazol-1-yl]ethoxy]ethoxy]ethyl 2-[3,6-bis(diethylamino)-9H-xanthen-9-yl]benzoate	non-polymer		803.9	3	Chemie (R3F)
4		water	water		18.0	150	Chemie (HOH)

Sequence modifications

A: 0 - 87 (UniProt: A0A0S4TLR1)

PDB	External Database	Details
Ala 17	Arg 18	engineered mutation

A: 95 - 178 (UniProt: A0A0S4TLR1)

PDB	External Database	Details
Ser 88	-	linker
Ser 89	-	linker
Thr 90	-	linker
Val 91	-	linker
Pro 92	-	linker
Gly 93	-	linker
Asp 94	-	linker
Ala 108	Arg 18	engineered mutation

A: 186 - 271 (UniProt: A0A0S4TLR1)

PDB	External Database	Details
Ser 179	-	linker
Ser 180	-	linker
Thr 181	-	linker
Val 182	-	linker
Pro 183	-	linker
Gly 184	-	linker
Asp 185	-	linker
Ala 199	Arg 18	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	29091.6
Non-Polymers*	Number of molecules	6
	Total formula weight	2688.1
All*	Total formula weight	31779.7

*Water molecules are not included.