7E7N
Crystal structure of RSL mutant-R17A/R108A/R199A in complex with R3F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-06-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.300, 45.635, 46.844 |
| Unit cell angles | 94.12, 117.04, 117.20 |
Refinement procedure
| Resolution | 39.040 - 1.550 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.20040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4csd |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.227 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.040 | 39.040 | 1.590 |
| High resolution limit [Å] | 1.500 | 4.760 | 1.500 |
| Rmerge | 0.043 | 0.026 | 0.480 |
| Rmeas | 0.051 | 0.031 | 0.565 |
| Rpim | 0.027 | 0.016 | 0.296 |
| Total number of observations | 143079 | 4501 | 21138 |
| Number of reflections | 40510 | 1292 | 5914 |
| <I/σ(I)> | 14.2 | 37.2 | 2.6 |
| Completeness [%] | 93.8 | 94.9 | 93.6 |
| Redundancy | 3.5 | 3.5 | 3.6 |
| CC(1/2) | 0.999 | 0.998 | 0.882 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 7.5 | 277 | 20 mM Tris-HCl, 100 mM of NaCl pH 7.5, Micro centrifuge tube sequentially put with RSL solution, pure buffer, the ligand solution |
