7E4R

Crystal structure of tubulin in complex with D-DM1-SMe

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Tubulin alpha-1B chain	polymer	440	48966.3	2	UniProt (P81947) Pfam (PF00091) Pfam (PF03953)	Bos taurus (Bovine)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B, D (B, D)	Tubulin beta-2B chain	polymer	431	48391.4	2	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953)	Bos taurus (Bovine)
3	E (E)	Stathmin-4	polymer	138	16282.5	1	UniProt (P63043) Pfam (PF00836)	Rattus norvegicus (Rat)	Stathmin-like protein B3,RB3
4	F (F)	Tubulin tyrosine ligase	polymer	380	43825.9	1	UniProt (E1BQ43)	Gallus gallus (Chicken)
5	G, M, P (A, C, D)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	3	Chemie (GTP)
6	H, K, N, R (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	I, O (A, C)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
8	J (B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
9	L (B)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
10	Q (D)	(10E,12E)-86-chloro-14-hydroxy-85,14-dimethoxy-33,2,7,10-tetramethyl-12,6-dioxo-7-aza-1(6,4)-oxazinana-3(2,3)-oxirana-8(1,3)-benzenacyclotetradecaphane-10,12-dien-4-yl N-methyl-N-(3-(methylsulfinothioyl)propanoyl)-D-alaninate	non-polymer		784.4	1	Chemie (HZ0)
11	S (F)	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP)
12	T, U, V, W, X... (A, B, C, D, E...)	water	water		18.0	322	Chemie (HOH)

Sequence modifications

F: 1 - 378 (UniProt: E1BQ43)

PDB	External Database	Details
His 379	-	expression tag
His 380	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	254823.9
Non-Polymers*	Number of molecules	13
	Total formula weight	3674.9
All*	Total formula weight	258498.8

*Water molecules are not included.