7E40

Mechanism of Phosphate Sensing and Signaling Revealed by Rice SPX1-PHR2 Complex Structure

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Protein PHOSPHATE STARVATION RESPONSE 2	polymer	134	15554.0	2	UniProt (Q6Z156) Pfam (PF00249) Pfam (PF14379) In PDB	Oryza sativa subsp. japonica (Rice)	OsPHR2
2	B, D	SPX domain-containing protein 1,Endolysin	polymer	358	40828.9	2	UniProt (Q69XJ0) UniProt (D9IEF7) Pfam (PF03105) Pfam (PF00959) In PDB	Oryza sativa subsp. japonica (Rice)	Protein SPX DOMAIN GENE 1,OsSPX1,Lysis protein,Lysozyme,Muramidase
3	B, D	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	3	Chemie (IHP)
4		water	water		18.0	17	Chemie (HOH)

Sequence modifications

A, C: 248 - 380 (UniProt: Q6Z156)

PDB	External Database	Details
Ser 247	-	expression tag
Met 263	Val 263	engineered mutation
Met 278	Leu 278	engineered mutation
Met 295	Leu 295	engineered mutation
Met 340	Leu 340	engineered mutation

B, D: 1 - 198 (UniProt: Q69XJ0)

PDB	External Database	Details
Ser 0	-	expression tag

B, D: 199 - 357 (UniProt: D9IEF7)

PDB	External Database	Details
Thr 250	Cys 54	engineered mutation
Ala 293	Cys 97	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	112765.9
Non-Polymers*	Number of molecules	3
	Total formula weight	1980.1
All*	Total formula weight	114746.0

*Water molecules are not included.