7E40

Mechanism of Phosphate Sensing and Signaling Revealed by Rice SPX1-PHR2 Complex Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	65.244, 107.492, 174.521
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.760 - 2.600
R-factor	0.2281
Rwork	0.226
R-free	0.27410
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3861)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.760	2.750
High resolution limit [Å]	2.600	2.600
Rmerge	0.099	0.715
Rmeas	0.107	0.775
Number of reflections	38730	11788
<I/σ(I)>	13.94	2.4
Completeness [%]	99.9	99.9
Redundancy	7.042	6.787
CC(1/2)	0.998	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	277.15	0.1M Sodium phosphate dibasic/Citric acid pH 4.2, 10% PEG 3350, 0.2M NH4HCO3