7E40
Mechanism of Phosphate Sensing and Signaling Revealed by Rice SPX1-PHR2 Complex Structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.244, 107.492, 174.521 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.760 - 2.600 |
R-factor | 0.2281 |
Rwork | 0.226 |
R-free | 0.27410 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18_3861) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.760 | 2.750 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.099 | 0.715 |
Rmeas | 0.107 | 0.775 |
Number of reflections | 38730 | 11788 |
<I/σ(I)> | 13.94 | 2.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.042 | 6.787 |
CC(1/2) | 0.998 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 277.15 | 0.1M Sodium phosphate dibasic/Citric acid pH 4.2, 10% PEG 3350, 0.2M NH4HCO3 |