7DVQ
Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pre-mRNA-processing-splicing factor 8 | polymer | 2335 | 273974.3 | 1 | UniProt (Q6P2Q9) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF01398) Pfam (PF08084) | Homo sapiens (Human) | 220 kDa U5 snRNP-specific protein,PRP8 homolog,Splicing factor Prp8,p220 |
| 2 | B (B) | U5 snRNA | polymer | 117 | 37254.9 | 1 | GenBank (20330981) | Homo sapiens | |
| 3 | C (C) | 116 kDa U5 small nuclear ribonucleoprotein component | polymer | 972 | 109560.6 | 1 | UniProt (Q15029) Pfam (PF16004) Pfam (PF00009) Pfam (PF03144) Pfam (PF14492) Pfam (PF03764) Pfam (PF00679) | Homo sapiens (Human) | Elongation factor Tu GTP-binding domain-containing protein 2,SNU114 homolog,hSNU114,U5 snRNP-specific protein,116 kDa,U5-116 kDa |
| 4 | D (D) | U5 small nuclear ribonucleoprotein 200 kDa helicase | polymer | 2136 | 244823.4 | 1 | UniProt (O75643) Pfam (PF21188) Pfam (PF18149) Pfam (PF00270) Pfam (PF00271) Pfam (PF23445) Pfam (PF02889) | Homo sapiens (Human) | Activating signal cointegrator 1 complex subunit 3-like 1,BRR2 homolog,U5 snRNP-specific 200 kDa protein,U5-200KD |
| 5 | E (E) | U5 small nuclear ribonucleoprotein 40 kDa protein | polymer | 357 | 39359.5 | 1 | UniProt (Q96DI7) Pfam (PF00400) | Homo sapiens (Human) | U5-40K,38 kDa-splicing factor,Prp8-binding protein,hPRP8BP,U5 snRNP-specific 40 kDa protein,WD repeat-containing protein 57 |
| 6 | F, P (a, h) | Small nuclear ribonucleoprotein Sm D3 | polymer | 126 | 13940.3 | 2 | UniProt (P62318) Pfam (PF01423) | Homo sapiens (Human) | Sm-D3,snRNP core protein D3 |
| 7 | G, Q (b, i) | Small nuclear ribonucleoprotein-associated proteins B and B' | polymer | 240 | 24642.1 | 2 | UniProt (P14678) Pfam (PF01423) | Homo sapiens (Human) | snRNP-B,Sm protein B/B',SmB/B' |
| 8 | H, R (c, j) | Small nuclear ribonucleoprotein Sm D1 | polymer | 119 | 13310.7 | 2 | UniProt (P62314) Pfam (PF01423) | Homo sapiens (Human) | Sm-D1,Sm-D autoantigen,snRNP core protein D1 |
| 9 | I, S (d, k) | Small nuclear ribonucleoprotein Sm D2 | polymer | 118 | 13551.9 | 2 | UniProt (P62316) Pfam (PF01423) | Homo sapiens (Human) | Sm-D2,snRNP core protein D2 |
| 10 | J, T (f, m) | Small nuclear ribonucleoprotein F | polymer | 86 | 9734.2 | 2 | UniProt (P62306) Pfam (PF01423) | Homo sapiens (Human) | snRNP-F,Sm protein F,SmF |
| 11 | K, U (e, l) | Small nuclear ribonucleoprotein E | polymer | 92 | 10817.6 | 2 | UniProt (P62304) Pfam (PF01423) | Homo sapiens (Human) | snRNP-E,Sm protein E,SmE |
| 12 | L, V (g, n) | Small nuclear ribonucleoprotein G | polymer | 76 | 8508.1 | 2 | UniProt (P62308) Pfam (PF01423) | Homo sapiens (Human) | snRNP-G,Sm protein G,SmG |
| 13 | M (F) | U6atac snRNA | polymer | 124 | 39876.6 | 1 | GenBank (187960111) | Homo sapiens | |
| 14 | N (G) | pre-mRNA | polymer | 142 | 44322.8 | 1 | Homo sapiens | ||
| 15 | O (H) | U12 snRNA | polymer | 150 | 48353.6 | 1 | GenBank (951206) | Homo sapiens | |
| 16 | W (v) | Sodium channel modifier 1 | polymer | 230 | 25993.5 | 1 | UniProt (Q9BWG6) Pfam (PF15803) Pfam (PF15805) | Homo sapiens (Human) | |
| 17 | X (1) | Splicing factor 3B subunit 1 | polymer | 1304 | 146104.9 | 1 | UniProt (O75533) Pfam (PF08920) Pfam (PF22646) | Homo sapiens (Human) | Pre-mRNA-splicing factor SF3b 155 kDa subunit,SF3b155,Spliceosome-associated protein 155,SAP 155 |
| 18 | Y (2) | Splicing factor 3B subunit 2 | polymer | 895 | 100377.8 | 1 | UniProt (Q13435) Pfam (PF02037) Pfam (PF04037) Pfam (PF04046) | Homo sapiens (Human) | Pre-mRNA-splicing factor SF3b 145 kDa subunit,SF3b145,Spliceosome-associated protein 145,SAP 145 |
| 19 | Z (3) | Splicing factor 3B subunit 3 | polymer | 1217 | 135798.8 | 1 | UniProt (Q15393) Pfam (PF10433) Pfam (PF23726) Pfam (PF03178) | Homo sapiens (Human) | Pre-mRNA-splicing factor SF3b 130 kDa subunit,SF3b130,STAF130,Spliceosome-associated protein 130,SAP 130 |
| 20 | AA (4) | Splicing factor 3B subunit 4 | polymer | 424 | 44436.6 | 1 | UniProt (Q15427) Pfam (PF00076) | Homo sapiens (Human) | Pre-mRNA-splicing factor SF3b 49 kDa subunit,Spliceosome-associated protein 49,SAP 49 |
| 21 | BA (5) | Splicing factor 3B subunit 6 | polymer | 125 | 14606.9 | 1 | UniProt (Q9Y3B4) Pfam (PF00076) | Homo sapiens (Human) | Pre-mRNA branch site protein p14,SF3b 14 kDa subunit,SF3B14a,Spliceosome-associated protein,14-kDa,Splicing factor 3b,subunit 6,14kDa |
| 22 | CA (6) | PHD finger-like domain-containing protein 5A | polymer | 110 | 12427.5 | 1 | UniProt (Q7RTV0) Pfam (PF03660) | Homo sapiens (Human) | PHD finger-like domain protein 5A,Splicing factor 3B-associated 14 kDa protein,SF3b14b |
| 23 | DA (7) | Splicing factor 3B subunit 5 | polymer | 86 | 10149.4 | 1 | UniProt (Q9BWJ5) Pfam (PF07189) | Homo sapiens (Human) | SF3b5,Pre-mRNA-splicing factor SF3b 10 kDa subunit |
| 24 | EA (L) | Cell division cycle 5-like protein | polymer | 802 | 92406.9 | 1 | UniProt (Q99459) Pfam (PF13921) Pfam (PF11831) | Homo sapiens (Human) | Cdc5-like protein,Pombe cdc5-related protein |
| 25 | FA (J) | Crooked neck-like protein 1 | polymer | 848 | 100610.0 | 1 | UniProt (Q9BZJ0) Pfam (PF23231) | Homo sapiens (Human) | Crooked neck homolog,hCrn |
| 26 | GA (P) | Spliceosome-associated protein CWC15 homolog | polymer | 229 | 26674.4 | 1 | UniProt (Q9P013) Pfam (PF04889) | Homo sapiens (Human) | |
| 27 | HA (R) | SNW domain-containing protein 1 | polymer | 536 | 61610.7 | 1 | UniProt (Q13573) Pfam (PF02731) | Homo sapiens (Human) | Nuclear protein SkiP,Nuclear receptor coactivator NCoA-62,Ski-interacting protein |
| 28 | IA (T) | Pleiotropic regulator 1 | polymer | 514 | 57280.8 | 1 | UniProt (O43660) Pfam (PF00400) | Homo sapiens (Human) | |
| 29 | JA (X) | Smad nuclear-interacting protein 1 | polymer | 396 | 45880.7 | 1 | UniProt (Q8TAD8) Pfam (PF00498) | Homo sapiens (Human) | FHA domain-containing protein SNIP1 |
| 30 | KA (Y) | RNA-binding motif protein, X-linked 2 | polymer | 322 | 37426.0 | 1 | UniProt (Q9Y388) Pfam (PF00076) | Homo sapiens (Human) | |
| 31 | LA (Z) | BUD13 homolog | polymer | 619 | 70669.2 | 1 | UniProt (Q9BRD0) Pfam (PF09736) | Homo sapiens (Human) | |
| 32 | MA (9) | RING-type E3 ubiquitin-protein ligase PPIL2 | polymer | 520 | 58910.4 | 1 | UniProt (Q13356) Pfam (PF00160) | Homo sapiens (Human) | CYC4,Cyclophilin-60,Cyclophilin-like protein Cyp-60,Cyp60,hCyP-60,Probable inactive peptidyl-prolyl cis-trans isomerase-like 2,PPIase,Rotamase PPIL2 |
| 33 | NA (z) | Peptidyl-prolyl cis-trans isomerase CWC27 homolog | polymer | 472 | 53941.2 | 1 | UniProt (Q6UX04) Pfam (PF00160) | Homo sapiens (Human) | PPIase CWC27,Antigen NY-CO-10,Serologically defined colon cancer antigen 10 |
| 34 | OA (x) | Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 | polymer | 1041 | 119443.6 | 1 | UniProt (O60231) Pfam (PF00270) Pfam (PF00271) Pfam (PF04408) Pfam (PF21010) Pfam (PF07717) | Homo sapiens (Human) | ATP-dependent RNA helicase #3,DEAH-box protein 16 |
| 35 | PA (y) | G-patch domain and KOW motifs-containing protein | polymer | 476 | 52299.7 | 1 | UniProt (Q92917) Pfam (PF12656) Pfam (PF00467) | Homo sapiens (Human) | G-patch domain-containing protein 5,Protein MOS2 homolog,Protein T54 |
| 36 | QA (M) | RING finger protein 113A | polymer | 343 | 38847.2 | 1 | UniProt (O15541) Pfam (PF00642) Pfam (PF13920) | Homo sapiens (Human) | Zinc finger protein 183 |
| 37 | RA (U) | Serine/arginine repetitive matrix protein 2 | polymer | 2752 | 300255.3 | 1 | UniProt (Q9UQ35) Pfam (PF08312) | Homo sapiens (Human) | 300 kDa nuclear matrix antigen,Serine/arginine-rich splicing factor-related nuclear matrix protein of 300 kDa,Ser/Arg-related nuclear matrix protein of 300 kDa,Splicing coactivator subunit SRm300,Tax-responsive enhancer element-binding protein 803,TaxREB803 |
| 38 | SA (V) | Pre-mRNA-splicing factor CWC22 homolog | polymer | 908 | 105646.6 | 1 | UniProt (Q9HCG8) Pfam (PF02854) Pfam (PF02847) | Homo sapiens (Human) | Nucampholin homolog,fSAPb |
| 39 | TA (8) | Serine/arginine repetitive matrix protein 1 | polymer | 904 | 102600.5 | 1 | UniProt (Q8IYB3) Pfam (PF01480) | Homo sapiens (Human) | SR-related nuclear matrix protein of 160 kDa,SRm160,Ser/Arg-related nuclear matrix protein |
| 40 | UA (0) | Cysteine-rich PDZ-binding protein | polymer | 101 | 11239.1 | 1 | UniProt (Q9P021) Pfam (PF10235) | Homo sapiens (Human) | Cysteine-rich interactor of PDZ three,Cysteine-rich interactor of PDZ3 |
| 41 | VA (I) | RNA-binding protein 48 | polymer | 367 | 41878.5 | 1 | UniProt (Q5RL73) | Homo sapiens (Human) | |
| 42 | WA (K) | Armadillo repeat-containing protein 7 | polymer | 198 | 21946.0 | 1 | UniProt (Q9H6L4) Pfam (PF00514) | Homo sapiens (Human) | |
| 43 | XA (A) | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 1 | Chemie (IHP) | |||
| 44 | YA (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 45 | AB, BB, CB, DB, ZA (F, C) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) PubChem (888) | |||
| 46 | EB, FB (F) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) PubChem (813) | |||
| 47 | GB (F) | 5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine | non-polymer | 537.2 | 1 | Chemie (G5J) | |||
| 48 | HB, IB, JB, KB, LB... (v, 6, M, 0) | ZINC ION | non-polymer | 65.4 | 7 | Chemie (ZN) | |||
| 49 | OB (C) | water | water | 18.0 | 3 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 49 |
| Total formula weight | 2915997.9 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 2378.0 | |
| All* | Total formula weight | 2918375.9 |
