7DVQ
Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pre-mRNA-processing-splicing factor 8 | polymer | 2335 | 273974.3 | 1 | UniProt (Q6P2Q9) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF01398) Pfam (PF08084) | Homo sapiens (Human) | 220 kDa U5 snRNP-specific protein,PRP8 homolog,Splicing factor Prp8,p220 |
| 2 | B (B) | U5 snRNA | polymer | 117 | 37254.9 | 1 | GenBank (20330981) | Homo sapiens | |
| 3 | C (C) | 116 kDa U5 small nuclear ribonucleoprotein component | polymer | 972 | 109560.6 | 1 | UniProt (Q15029) Pfam (PF16004) Pfam (PF00009) Pfam (PF03144) Pfam (PF14492) Pfam (PF03764) Pfam (PF00679) | Homo sapiens (Human) | Elongation factor Tu GTP-binding domain-containing protein 2,SNU114 homolog,hSNU114,U5 snRNP-specific protein,116 kDa,U5-116 kDa |
| 4 | D (D) | U5 small nuclear ribonucleoprotein 200 kDa helicase | polymer | 2136 | 244823.4 | 1 | UniProt (O75643) Pfam (PF21188) Pfam (PF18149) Pfam (PF00270) Pfam (PF00271) Pfam (PF02889) | Homo sapiens (Human) | Activating signal cointegrator 1 complex subunit 3-like 1,BRR2 homolog,U5 snRNP-specific 200 kDa protein,U5-200KD |
| 5 | E (E) | U5 small nuclear ribonucleoprotein 40 kDa protein | polymer | 357 | 39359.5 | 1 | UniProt (Q96DI7) Pfam (PF00400) | Homo sapiens (Human) | U5-40K,38 kDa-splicing factor,Prp8-binding protein,hPRP8BP,U5 snRNP-specific 40 kDa protein,WD repeat-containing protein 57 |
| 6 | F, P (a, h) | Small nuclear ribonucleoprotein Sm D3 | polymer | 126 | 13940.3 | 2 | UniProt (P62318) Pfam (PF01423) | Homo sapiens (Human) | Sm-D3,snRNP core protein D3 |
| 7 | G, Q (b, i) | Small nuclear ribonucleoprotein-associated proteins B and B' | polymer | 240 | 24642.1 | 2 | UniProt (P14678) Pfam (PF01423) | Homo sapiens (Human) | snRNP-B,Sm protein B/B',SmB/B' |
| 8 | H, R (c, j) | Small nuclear ribonucleoprotein Sm D1 | polymer | 119 | 13310.7 | 2 | UniProt (P62314) Pfam (PF01423) | Homo sapiens (Human) | Sm-D1,Sm-D autoantigen,snRNP core protein D1 |
| 9 | I, S (d, k) | Small nuclear ribonucleoprotein Sm D2 | polymer | 118 | 13551.9 | 2 | UniProt (P62316) Pfam (PF01423) | Homo sapiens (Human) | Sm-D2,snRNP core protein D2 |
| 10 | J, T (f, m) | Small nuclear ribonucleoprotein F | polymer | 86 | 9734.2 | 2 | UniProt (P62306) Pfam (PF01423) | Homo sapiens (Human) | snRNP-F,Sm protein F,SmF |
| 11 | K, U (e, l) | Small nuclear ribonucleoprotein E | polymer | 92 | 10817.6 | 2 | UniProt (P62304) Pfam (PF01423) | Homo sapiens (Human) | snRNP-E,Sm protein E,SmE |
| 12 | L, V (g, n) | Small nuclear ribonucleoprotein G | polymer | 76 | 8508.1 | 2 | UniProt (P62308) Pfam (PF01423) | Homo sapiens (Human) | snRNP-G,Sm protein G,SmG |
| 13 | M (F) | U6atac snRNA | polymer | 124 | 39876.6 | 1 | GenBank (187960111) | Homo sapiens | |
| 14 | N (G) | pre-mRNA | polymer | 142 | 44322.8 | 1 | Homo sapiens | ||
| 15 | O (H) | U12 snRNA | polymer | 150 | 48353.6 | 1 | GenBank (951206) | Homo sapiens | |
| 16 | W (v) | Sodium channel modifier 1 | polymer | 230 | 25993.5 | 1 | UniProt (Q9BWG6) Pfam (PF15803) Pfam (PF15805) | Homo sapiens (Human) | |
| 17 | X (1) | Splicing factor 3B subunit 1 | polymer | 1304 | 146104.9 | 1 | UniProt (O75533) Pfam (PF08920) Pfam (PF22646) | Homo sapiens (Human) | Pre-mRNA-splicing factor SF3b 155 kDa subunit,SF3b155,Spliceosome-associated protein 155,SAP 155 |
| 18 | Y (2) | Splicing factor 3B subunit 2 | polymer | 895 | 100377.8 | 1 | UniProt (Q13435) Pfam (PF02037) Pfam (PF04037) Pfam (PF04046) | Homo sapiens (Human) | Pre-mRNA-splicing factor SF3b 145 kDa subunit,SF3b145,Spliceosome-associated protein 145,SAP 145 |
| 19 | Z (3) | Splicing factor 3B subunit 3 | polymer | 1217 | 135798.8 | 1 | UniProt (Q15393) Pfam (PF10433) Pfam (PF03178) | Homo sapiens (Human) | Pre-mRNA-splicing factor SF3b 130 kDa subunit,SF3b130,STAF130,Spliceosome-associated protein 130,SAP 130 |
| 20 | AA (4) | Splicing factor 3B subunit 4 | polymer | 424 | 44436.6 | 1 | UniProt (Q15427) Pfam (PF00076) | Homo sapiens (Human) | Pre-mRNA-splicing factor SF3b 49 kDa subunit,Spliceosome-associated protein 49,SAP 49 |
| 21 | BA (5) | Splicing factor 3B subunit 6 | polymer | 125 | 14606.9 | 1 | UniProt (Q9Y3B4) Pfam (PF00076) | Homo sapiens (Human) | Pre-mRNA branch site protein p14,SF3b 14 kDa subunit,SF3B14a,Spliceosome-associated protein,14-kDa,Splicing factor 3b,subunit 6,14kDa |
| 22 | CA (6) | PHD finger-like domain-containing protein 5A | polymer | 110 | 12427.5 | 1 | UniProt (Q7RTV0) Pfam (PF03660) | Homo sapiens (Human) | PHD finger-like domain protein 5A,Splicing factor 3B-associated 14 kDa protein,SF3b14b |
| 23 | DA (7) | Splicing factor 3B subunit 5 | polymer | 86 | 10149.4 | 1 | UniProt (Q9BWJ5) Pfam (PF07189) | Homo sapiens (Human) | SF3b5,Pre-mRNA-splicing factor SF3b 10 kDa subunit |
| 24 | EA (L) | Cell division cycle 5-like protein | polymer | 802 | 92406.9 | 1 | UniProt (Q99459) Pfam (PF13921) Pfam (PF11831) | Homo sapiens (Human) | Cdc5-like protein,Pombe cdc5-related protein |
| 25 | FA (J) | Crooked neck-like protein 1 | polymer | 848 | 100610.0 | 1 | UniProt (Q9BZJ0) Pfam (PF02184) | Homo sapiens (Human) | Crooked neck homolog,hCrn |
| 26 | GA (P) | Spliceosome-associated protein CWC15 homolog | polymer | 229 | 26674.4 | 1 | UniProt (Q9P013) Pfam (PF04889) | Homo sapiens (Human) | |
| 27 | HA (R) | SNW domain-containing protein 1 | polymer | 536 | 61610.7 | 1 | UniProt (Q13573) Pfam (PF02731) | Homo sapiens (Human) | Nuclear protein SkiP,Nuclear receptor coactivator NCoA-62,Ski-interacting protein |
| 28 | IA (T) | Pleiotropic regulator 1 | polymer | 514 | 57280.8 | 1 | UniProt (O43660) Pfam (PF00400) | Homo sapiens (Human) | |
| 29 | JA (X) | Smad nuclear-interacting protein 1 | polymer | 396 | 45880.7 | 1 | UniProt (Q8TAD8) Pfam (PF00498) | Homo sapiens (Human) | FHA domain-containing protein SNIP1 |
| 30 | KA (Y) | RNA-binding motif protein, X-linked 2 | polymer | 322 | 37426.0 | 1 | UniProt (Q9Y388) Pfam (PF00076) | Homo sapiens (Human) | |
| 31 | LA (Z) | BUD13 homolog | polymer | 619 | 70669.2 | 1 | UniProt (Q9BRD0) Pfam (PF09736) | Homo sapiens (Human) | |
| 32 | MA (9) | RING-type E3 ubiquitin-protein ligase PPIL2 | polymer | 520 | 58910.4 | 1 | UniProt (Q13356) Pfam (PF00160) | Homo sapiens (Human) | CYC4,Cyclophilin-60,Cyclophilin-like protein Cyp-60,Cyp60,hCyP-60,Probable inactive peptidyl-prolyl cis-trans isomerase-like 2,PPIase,Rotamase PPIL2 |
| 33 | NA (z) | Peptidyl-prolyl cis-trans isomerase CWC27 homolog | polymer | 472 | 53941.2 | 1 | UniProt (Q6UX04) Pfam (PF00160) | Homo sapiens (Human) | PPIase CWC27,Antigen NY-CO-10,Serologically defined colon cancer antigen 10 |
| 34 | OA (x) | Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 | polymer | 1041 | 119443.6 | 1 | UniProt (O60231) Pfam (PF00270) Pfam (PF00271) Pfam (PF04408) Pfam (PF21010) Pfam (PF07717) | Homo sapiens (Human) | ATP-dependent RNA helicase #3,DEAH-box protein 16 |
| 35 | PA (y) | G-patch domain and KOW motifs-containing protein | polymer | 476 | 52299.7 | 1 | UniProt (Q92917) Pfam (PF12656) Pfam (PF00467) | Homo sapiens (Human) | G-patch domain-containing protein 5,Protein MOS2 homolog,Protein T54 |
| 36 | QA (M) | RING finger protein 113A | polymer | 343 | 38847.2 | 1 | UniProt (O15541) Pfam (PF00642) Pfam (PF13920) | Homo sapiens (Human) | Zinc finger protein 183 |
| 37 | RA (U) | Serine/arginine repetitive matrix protein 2 | polymer | 2752 | 300255.3 | 1 | UniProt (Q9UQ35) Pfam (PF08312) | Homo sapiens (Human) | 300 kDa nuclear matrix antigen,Serine/arginine-rich splicing factor-related nuclear matrix protein of 300 kDa,Ser/Arg-related nuclear matrix protein of 300 kDa,Splicing coactivator subunit SRm300,Tax-responsive enhancer element-binding protein 803,TaxREB803 |
| 38 | SA (V) | Pre-mRNA-splicing factor CWC22 homolog | polymer | 908 | 105646.6 | 1 | UniProt (Q9HCG8) Pfam (PF02854) Pfam (PF02847) | Homo sapiens (Human) | Nucampholin homolog,fSAPb |
| 39 | TA (8) | Serine/arginine repetitive matrix protein 1 | polymer | 904 | 102600.5 | 1 | UniProt (Q8IYB3) Pfam (PF01480) | Homo sapiens (Human) | SR-related nuclear matrix protein of 160 kDa,SRm160,Ser/Arg-related nuclear matrix protein |
| 40 | UA (0) | Cysteine-rich PDZ-binding protein | polymer | 101 | 11239.1 | 1 | UniProt (Q9P021) Pfam (PF10235) | Homo sapiens (Human) | Cysteine-rich interactor of PDZ three,Cysteine-rich interactor of PDZ3 |
| 41 | VA (I) | RNA-binding protein 48 | polymer | 367 | 41878.5 | 1 | UniProt (Q5RL73) | Homo sapiens (Human) | |
| 42 | WA (K) | Armadillo repeat-containing protein 7 | polymer | 198 | 21946.0 | 1 | UniProt (Q9H6L4) Pfam (PF00514) | Homo sapiens (Human) | |
| 43 | XA (A) | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 1 | Chemie (IHP) | |||
| 44 | YA (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 45 | AB, BB, CB, DB, ZA (F, C) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 46 | EB, FB (F) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 47 | GB (F) | 5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine | non-polymer | 537.2 | 1 | Chemie (G5J) | |||
| 48 | HB, IB, JB, KB, LB... (v, 6, M, 0) | ZINC ION | non-polymer | 65.4 | 7 | Chemie (ZN) | |||
| 49 | OB (C) | water | water | 18.0 | 3 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 49 |
| Total formula weight | 2915997.9 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 2378.0 | |
| All* | Total formula weight | 2918375.9 |
