7DUW

Cryo-EM structure of the multiple peptide resistance factor (MprF) loaded with two lysyl-phosphatidylglycerol molecules

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Bifunctional lysylphosphatidylglycerol flippase/synthetase MprF	polymer	882	96094.7	2	UniProt (A0A6P1C618) Pfam (PF09924) In PDB	Rhizobium tropici
2	A, B	DODECYL-BETA-D-MALTOSIDE	non-polymer		510.6	2	Chemie (LMT)
3	A, B	[(2~{R})-3-[[(2~{S})-3-[(2~{S})-2,6-bis(azanyl)hexanoyl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-2-hexadecanoyloxy-propyl] hexadecanoate	non-polymer		851.1	4	Chemie (EV9)
4	A, B	1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE	non-polymer		723.0	2	Chemie (LHG)
5	A, B	(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE	non-polymer		751.0	2	Chemie (PGT)
6	A, B	(2~{R},3~{S},4~{S},5~{S},6~{S})-2-(hydroxymethyl)-6-[(2~{R},3~{S},4~{R},5~{R},6~{R})-2-(hydroxymethyl)-6-[2-[[(2~{R},3~{S},4~{R},5~{R},6~{S})-6-(hydroxymethyl)-5-[(2~{S},3~{R},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,4-bis(oxidanyl)oxan-2-yl]oxymethyl]-4-[(1~{R},2~{R},4~{S},5'~{R},6~{R},7~{R},8~{R},9~{S},12~{S},13~{R},16~{S})-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane]-16-yl]oxy-butoxy]-4,5-bis(oxidanyl)oxan-3-yl]oxy-oxane-3,4,5-triol	non-polymer		1165.3	2	Chemie (J4U)
7		water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, B: 1 - 869 (UniProt: A0A6P1C618)

PDB	External Database	Details
Lys 870	-	expression tag
Leu 871	-	expression tag
Ala 872	-	expression tag
Ala 873	-	expression tag
Ala 874	-	expression tag
Leu 875	-	expression tag
Glu 876	-	expression tag
His 877	-	expression tag
His 878	-	expression tag
His 879	-	expression tag
His 880	-	expression tag
His 881	-	expression tag
His 882	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	192189.3
Non-Polymers*	Number of molecules	12
	Total formula weight	9704.4
All*	Total formula weight	201893.8

*Water molecules are not included.