7DUW

Cryo-EM structure of the multiple peptide resistance factor (MprF) loaded with two lysyl-phosphatidylglycerol molecules

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TALOS ARCTICA
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Cs (mm)	2.7
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.96
Number of Particle	1232621
Software	cryoSPARC,RELION,PHENIX,SerialEM,CTFFIND,Coot,UCSF Chimera
Symmetry	C2
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION